Alternating temperature (22°C / -1°C) pretreatments were effective for
increasing cold hardiness and recovery of cryopreserved meristems. Combining low
temperature pretreatment with abscisic acid (ABA) or sucrose treatments reduced the
time required for optimal cold hardiness of meristems. Desiccation tolerance was
directly correlated with increased cold hardiness and recovery of...
Roots of greenhouse-grown peppermint (Mentha piperita L.) and spearmint (Mentha spicata L.)
plants and in vitro-grown shoot cultures were inoculated with Verticillium dahliae conidial suspensions to
study symptom development, detection, and elimination of the V. dahliae. There were significant
differences in the symptom expression of control and infected shoot cultures...
Improved recovery of cryopreserved meristems and calli from in
vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants
was obtained by two-hour pretreatment with sucrose, proline, abscisic
acid-responsive protein (RABP), and bovine serum albumin (BSA)
solutions. Meristems and calli cold-acclimatized for one week were
immersed in pretreatment...
Bacterial contamination is one of the most important problems
both in plant tissue culture research and commercial laboratories. Early
detection of contaminants saves time, effort, and money. Strawberry
runner explants were screened for contaminants during June to August,
1994 and 1995. Most contaminants found were bacterial rather than
The effect of increased manganese superoxide dismutase (Mn-SOD) and
cytosolic ascorbate peroxidase (cAPX) on various abiotic stresses was studied
using transgenic tomato (Lycopersicon esculentum) plants. Transformants were
selected in vitro based on kanamycin resistance, confirmed by PCR and northern
blot analyses. Northern blots showed an enhanced gene expression of Mn-SOD...
Four aspects of factors influencing the accuracy of nondestructive chlorophyll (Chl) and nitrogen (N) measurement in fresh leaves were studied: (1) optimum wavelength (OW) identification; (2) indices development and evaluation; (3) influence of leaf properties; and (4) influence of meter parameters and sampling technique. Results were used to develop indices...