Index Catalog // ScholarsArchive@OSU

Characterization of the Sodium Transporting NADH:Quinone Oxidoreductase (NQR) in Vibrio cholerae

Creator:: Fassio, Sara R.
Abstract:: Vibrio cholerae is the causative agent of cholera, a serious diarrheal disease in developing countries. V. cholerae has a unique redox driven respiration-linked sodium pump, Na⁺ translocating NADH:quinone oxidoreductase (NQR). Several reports previously showed that NQR plays an important role in virulence, metabolism, and sodium homeostasis of V. cholerae. This...
Resource Type:: Honors College Thesis
Full Text:: goal was to characterize the role of NQR on V. cholerae virulence gene expression, metabolism, and

Roles of the Sodium-Translocating NADH:Quinone Oxidoreductase (Na⁺-NQR) on Vibrio cholerae Metabolism, Motility and Osmotic Stress Resistance

Creator:: Minato, Yusuke, Fassio, Sara R., Kirkwood, Jay S., Halang, Petra, Quinn, Matthew J., Faulkner, Wyatt J., Aagesen, Alisha M., Steuber, Julia, Stevens, Jan F., and Häse, Claudia C.
Abstract:: The Na⁺ translocating NADH:quinone oxidoreductase (Na⁺-NQR) is a unique respiratory enzyme catalyzing the electron transfer from NADH to quinone coupled with the translocation of sodium ions across the membrane. Typically, Vibrio spp., including Vibrio cholerae, have this enzyme but lack the proton-pumping NADH:ubiquinone oxidoreductase (Complex I). Thus, Na⁺-NQR should significantly...
Resource Type:: Article

Malonate Inhibits Virulence Gene Expression in Vibrio cholerae

Creator:: Minato, Yusuke, Fassio, Sara R., and Häse, Claudia C.
Abstract:: We previously found that inhibition of the TCA cycle, either through mutations or chemical inhibition, increased toxT transcription in Vibrio cholerae. In this study, we found that the addition of malonate, an inhibitor of succinate dehydrogenase (SDH), decreased toxT transcription in V. cholerae, an observation inconsistent with the previous pattern...
Resource Type:: Article
Full Text:: Figure 1. Effects of growth conditions on virulence gene transcriptions. V. cholerae toxT::lacZ (A and B

Molecular and genetic assessment of selected antiporters and methyl-accepting chemotaxis proteins in Vibrio cholerae

Creator:: Quinn, Matthew J.
Abstract:: The pathogen Vibrio cholerae uses cations as a primary currency of virulence and environmental persistence, using gradients of those cations to move, acquire nutrients, and control virulence gene expression. An understanding of the overlapping roles of bioenergetics and chemotaxis in the virulence and environmental survival of V. cholerae issues from...
Resource Type:: Dissertation
Full Text:: C. Häse The pathogen Vibrio cholerae uses cations as a primary currency of virulence and

FassioSaraMicrobiologyRolesSodium-Translocating.pdf

Creator:: Minato, Yusuke, Fassio, Sara R., Kirkwood, Jay S., Halang, Petra, Quinn, Matthew J., Faulkner, Wyatt J., Aagesen, Alisha M., Steuber, Julia, Stevens, Jan F., and Häse, Claudia C.
Abstract:: The Na⁺ translocating NADH:quinone oxidoreductase (Na⁺-NQR) is a unique respiratory enzyme catalyzing the electron transfer from NADH to quinone coupled with the translocation of sodium ions across the membrane. Typically, Vibrio spp., including Vibrio cholerae, have this enzyme but lack the proton-pumping NADH:ubiquinone oxidoreductase (Complex I). Thus, Na⁺-NQR should significantly...
Full Text:: Roles of the Sodium-Translocating NADH:Quinone Oxidoreductase (Na [superscript +]-NQR) on Vibrio

HaeseClaudiaVeterinaryMedicineInhibitionSodiumTranslocating.pdf

Creator:: Minato, Yusuke, Fassio, Sara R., Reddekopp, Rylan L., and Häse, Claudia C.
Abstract:: Two virulence factors produced by Vibrio cholerae, cholera toxin (CT) and toxin-corregulated pilus (TCP), are indispensable for cholera infection. ToxT is the central regulatory protein involved in activation of CT and TCP expression. We previously reported that lack of a respiration-linked sodium-translocating NADH–ubiquinone oxidoreductase (Na⁺-NQR) significantly increases toxT transcription. In...
Full Text:: exponential growth phase Inhibition of the sodium-translocating NADH-ubiquinone oxidoreductase [Na+-NQR

Role of Na+/H+ Membrane Bioenergetics in Virulence Factors of Pseudomonas aeruginosa

Creator:: Hoang, Kelli H.
Abstract:: Pseudomonas aeruginosa is a bacterial pathogen that can cause grave and sometimes chronic infections in patients with weakened immune systems and cystic fibrosis. It is suspected that specialized sodium pumps in the cellular membrane are crucial for the organism’s survival and growth, since many cellular processes rely on the maintenance...
Resource Type:: Honors College Thesis
Full Text:: nuo gene, had some consistent impact on the organism’s growth regardless of the pH and sodium

FassioSaraMicrobiologyRolesSodium-Translocating_SupportingInformation.xlsx

Creator:: Minato, Yusuke, Fassio, Sara R., Kirkwood, Jay S., Halang, Petra, Quinn, Matthew J., Faulkner, Wyatt J., Aagesen, Alisha M., Steuber, Julia, Stevens, Jan F., and Häse, Claudia C.
Abstract:: The Na⁺ translocating NADH:quinone oxidoreductase (Na⁺-NQR) is a unique respiratory enzyme catalyzing the electron transfer from NADH to quinone coupled with the translocation of sodium ions across the membrane. Typically, Vibrio spp., including Vibrio cholerae, have this enzyme but lack the proton-pumping NADH:ubiquinone oxidoreductase (Complex I). Thus, Na⁺-NQR should significantly...
Full Text:: Sheet1 Plate Well Difference Substrate Mode of action PM07 A02 -143

ASM2012_NQR_Posterfinal-1.pptx

Creator:: Fassio, Sara R., Minato, Yusuke, Quinn, Matthew J., Faulkner, Wyatt J., and Hase, Claudia C.
Abstract:: We previously reported that inhibition of the Na⁺ translocating NADH:ubiquinone oxidoreductase (NQR), either by chemical inhibition or mutation, increased toxT transcription in Vibrio cholerae. In this study, we revealed that the nqr mutant strain showed similar phenotypes as the Escherichia coli NADH dehydrogenase I (nuo) mutant strain (e.g. growth defect...
Full Text:: role for NQR in V. cholerae metabolism. The effects of NQR on virulence gene expression are growth

Iron acquisition and its regulation in Nitosomonas europaea

Creator:: Vajrala, Neeraja
Abstract:: Iron is absolutely necessary for the maintenance, the defense, the differentiation and last, but by no means least, the growth and cellular division of almost all living organisms including Nitrosomonas europaea, an ammonia oxidizing bacterium. Given the necessity of iron, it is not surprising that N. europaea evolved several irondependent...
Resource Type:: Dissertation
Full Text:: fur homolog in iron metabolism and gene regulation in Nitrosomonas europaea…………………………………………118

ScholarsArchive@OSU

Characterization of the Sodium Transporting NADH:Quinone Oxidoreductase (NQR) in Vibrio cholerae

Roles of the Sodium-Translocating NADH:Quinone Oxidoreductase (Na⁺-NQR) on Vibrio cholerae Metabolism, Motility and Osmotic Stress Resistance

Malonate Inhibits Virulence Gene Expression in Vibrio cholerae

Molecular and genetic assessment of selected antiporters and methyl-accepting chemotaxis proteins in Vibrio cholerae

FassioSaraMicrobiologyRolesSodium-Translocating.pdf

HaeseClaudiaVeterinaryMedicineInhibitionSodiumTranslocating.pdf

Role of Na+/H+ Membrane Bioenergetics in Virulence Factors of Pseudomonas aeruginosa

FassioSaraMicrobiologyRolesSodium-Translocating_SupportingInformation.xlsx

ASM2012_NQR_Posterfinal-1.pptx

Iron acquisition and its regulation in Nitosomonas europaea

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