An Ls-isocitrate:NADP oxidoreductase has been isolated and
purified from embryonic chicken liver. The enzyme was purified
200 fold by means of ammonium sulfate fractionation, gel filtration
chromatography on Sephadex G-200, ion-exchange chromatography
on Bio-Gel CM-100 and adsorption chromatography on hydroxylapatite
gel.
The purified enzyme was nearly homogeneous as determined
from...
Maternally-derived transcripts and proteins support early bovine and ovine embryo development until the 8- to 16-cell stage, at which time embryonic transcripts become essential for continued development. One purported mechanism for the switch from maternal to zygotic control of development (maternal to zygotic genome activation; MZGA) is the appearance of...
The role of synthesis and degradation in controlling enzyme
concentrations was studied.
Concentrations of chloride and arginine were determined for
blood and liver at various stages of development from the embryo to
the 8 day old chicken. The chloride concentrations were used to
obtain the volume of extracellular blood in...
In the first experiment, estradiol production was measured in cultured embryos collected on Days 7, 9, 11, 13 and 15 post-mating. Estradiol was detected in Day 7 embryos and production increased approximately 50-fold (P<0.05) in medium recovered from Day 13 compared to Day 11 embryos and was greatest (P<0.05) from...
Myelin formation in the chick embryo sciatic nerve was examined
during development by analysis of three myelin lipids (cholesterol,
phospholipids, and cerebrosides) and of a myelin related
enzyme (2', 3'-cyclic AMP 3'-phosphohydrolase). The role of the
Schwann cell in controlling the synthesis of these myelin components
was studied by in...
The concept of genetic penetrance, "the frequency of manifestation of a genetic factor," was introduced by Timofeef-Ressovsky (Naturwissenschaften 19:493,1931). Incomplete penetrance has been used to explain the absence of phenotypic expression when otherwise anticipated. Studies of Embryonic Chick Edema, ECE (Poultry Sci. 77(suppl. 1):69, 1998) have been conducted in order...
Sodium dodecyl sulfate polyacrylamide gel electrophoresis and
zymography were used in two separate experiments to determine the tissue
source and type of plasminogen activator (PA) produced by bovine
blastocysts. Twelve-14 d blastocysts were collected at slaughter from
estrous synchronized, superovulated and artificially inseminated Holstein
cows. In Experiment I, blastocysts were...