Interest by public health organizations in scaling-up efficacious health interventions is increasing as the prevalence of arthritis increases and treatment becomes more costly. Although arthritis evidence-based interventions (EBIs) have been found efficacious in improving healthy behaviors and the ability to self-manage symptoms, little research has examined whether scaled-up delivery of...
Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate
sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and
genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe
conditional alleles of the dicer-related helicase, drh-3, that abrogate both RNA interference and...
The partitioning of body fluids in the Pacific hagfish was investigated
using several experimental techniques. Direct measurement
of the blood volume was obtained through the simultaneous use
of tagged red blood cells and plasma proteins labeled with Evan's
blue dye. Blood cells obtained from donor animals were incubated
with L-methionin-methyl-C¹⁴...
A method for isolating the salt gland (SG) from the larval brine
shrimp has been developed. This extra-renal organ is removed from 5-10%
of Stage Inauplii by vortexing after incubating the animals at 37°C for
8 hours in a saline solution buffered with 100 mM sodium phosphate at pH
7.6....
Characterization of the enzymatic activity of the ionocyte
(mitochondria-rich cell) in the gill epithelium of Oncorhynchus
tshawytscha was explored.
1. A method for estimating the enzymatic activity per cell for
tissues is described.
2. The DNA content per cell for nucleated erythrocytes (O. tshawytscha) was determined to be 9.47 x...
The cellular dynamics of the intestinal epithelium in the coho
salmon, Oncorhynchus kisutch, has been studied to determine the effects of temperature and whole-body X-irradiation.
In vivo experiments utilizing colchicine mitotic inhibiting technique and tritiated
thymidine (³H-TdR) radioautography were employed in order to
evaluate the kinetics of the cell population....
Effects of the marine environment on age and size at maturity, early marine growth, and abundance of chum salmon, Oncorhynchus keta, were studied at Olsen Creek during 1959-77. Chum salmon returned to Olsen Creek as predominately 3-, 4-, and 5-year fish; however, age composition varied from year to year. The...
By using immobilized trypsin, I have been able to
prepare well-defined, stable trypsinized nucleosomes. The
difficulties of lacking of control in the extent of
trypsinization, which were encountered in previous studies
with the use of free trypsin, have been eliminated. The
nucleosomes and oligonucleosomes prepared by immobilized
trypsin are suitable...
In most technical fields, a certain amount of practical application is necessary to master the important concepts and acquire basic problem solving skills. Computer science is no different, and this is reflected in the frequent programming assignments given in programming and data structures courses. The basic skills and concepts gained...