Crown gall disease is caused by the ubiquitous soil bacterium Agrobacterium tumefaciens which transfers a portion if DNA (T-DNA) into the plant cell. Preventing infection by using the biocontrol strain Agrobacterium radiobacter K84 is currently the only defense for crown gall. Two different resistance strategies were examined in this work....
Using combined HPLC-RIA, cytokinin levels in a series of tobacco crown gall tumors, having altered morphologies as a result of transposon mutagenesis were examined. Average trans-ribosylzeatin levels in the shoot-producing tumors (tms mutants) and root-producing tumors (tmr mutants) were 1400 pmol/gm and 0.97 pmol/gm, respectively. Cytokinin/auxin ratios were highest in...
Agrobacterium tumefaciens is a gram-negative soil bacterium
that causes crown gall tumors on dicotyledenous plants. The
transferred DNA (T-DNA) portion of the A. tumefaciens tumor-inducing
(Ti) plasmid enters infected plant cells and integrates into
plant nuclear DNA. The T-DNA is accompanied into plant cells by the
VirD2 endonuclease covalently attached...
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