Correction: 7 Mar 2016: The PLOS ONE Staff (2016) Correction: Successful Recovery of Nuclear Protein-Coding Genes from Small Insects in Museums Using Illumina Sequencing. PLOS ONE 11(3): e0151124. https://doi.org/10.1371/journal.pone.0151124
The advent of high-throughput sequencing (HTS) methods has enabled direct approaches to quantitatively profile small RNA
populations. However, these methods have been limited by several factors, including representational artifacts and lack of
established statistical methods of analysis. Furthermore, massive HTS data sets present new problems related to data processing
and...
To detect and avoid illegal logging of valuable tree species, identification methods for the origin of timber are necessary. We used next-generation sequencing to identify chloroplast genome regions that differentiate the origin of white oaks from the three continents; Asia, Europe, and North America. By using the chloroplast genome of...
Horizontal gene transfer (HGT) of DNA from the plastid to the nuclear and mitochondrial genomes of higher plants is a common
phenomenon; however, plastid genomes (plastomes) are highly conserved and have generally been regarded as impervious to HGT.
We sequenced the 158 kb plastome and the 690 kb mitochondrial genome...
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(Apocynaceae)
Shannon C.K. Straub1,*, Richard C. Cronn2, Christopher Edwards1, Mark Fishbein3, and Aaron
The natural production of methane via anaerobic digestion (AD) is facilitated by a complex community of microorganisms, primarily bacteria and archaea, referred to as the AD microbiome. AD is growing in prominence as a solution to the disposal of food waste, sewage sludge, and the organic fraction of municipal solid...
Further enhancement of energy generation is desired for practical application of anaerobic microbial systems such as microbial fuel cells (MFC) and anaerobic digesters (AD). A possible approach is to enhance the ability of microbial communities to transfer electron extracellularly in the form of electrical current. Critical to perform direct extracellular...
PREMISE OF THE STUDY: Hyb-Seq, the combination of target enrichment and genome skimming
allows simultaneous data collection for low-copy nuclear genes and high-copy genomic targets
for plant systematics and evolution studies.
METHODS AND RESULTS: Genome and transcriptome assemblies for milkweed (Asclepias syriaca)
were utilized to design enrichment probes for 3385...
Lung cancer in humans is an adult-onset disease. It is the number one cause of cancer mortality for both men and women and has a poor (15%) survival rate. Biological markers of the disease would allow for early diagnosis and treatment. The specific aims of this research project were to...
BACKGROUND: Cell types are defined at the molecular level during embryogenesis by a process called pattern formation and created by the selective utilization of combinations of sequence-specific transcription factors. Developmental programs define the sets of genes that are available to each particular cell type, and real-time biochemical signaling interactions define...
Cyanophage are viruses that infect cyanobacteria, photosynthetic microorganisms which are known to produce several anticancer and antibacterial chemical compounds. Cyanophage are significant members of microbial communities in marine and freshwater environments. There has been significant difficulty inventorying marine and freshwater cyanophage, however, due to the difficulty in cultivating their hosts....
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sequencing preparation and technical expertise, MarkDasenko of
the Center for Genome Research and