The Escherichia coli sulfur starvation utilization (ssu) operon includes a two-component monooxygenase system made up of an NADPH-dependent FMN reductase, SsuE, and a monooxygenase, SsuD. SsuE is part of the flavodoxin-like superfamily, and we report here the crystal structures of its apo, FMN-bound and FMNH₂-bound forms at ~2 Å resolution....
Sulfur is one of the six elements required during the early stages of the evolution of life, and enzymes involved in sulfur transfer and oxidation are increasingly being recognized as potential drug targets for antimicrobials as well as for therapies for cancer, neurodegenerative and inflammatory diseases. Bacteria are able to...
There is a growing need to establish alternative approaches for assessing hazard from chemical exposure compared to the traditional method of animal testing. Approaches that relate chemical structure to biological activity are gaining acceptance in the field as in silico methods for characterizing and predicting chemical toxicity. In this study,...
RNA sequencing (RNA-seq) and genotyping-by-sequencing (GBS) were used for single nucleotide polymorphism (SNP) identification from two economically important obligate plant pathogens, Pseudoperonospora cubensis and P. humuli. Twenty isolates of P. cubensis and 19 isolates of P. humuli were genotyped using RNA-seq and GBS. Principle components analysis (PCA) of each data...
Display, which is the function for displaying the
schema of the network at the conceptual level, is implemented
to enhance the network database system ALLEGRO.
To display the schema is one step towards modifiable
schemas in the network model. The problem of updating a
schema is examined at all three...
Emerging data showing the presence of atmospheric microplastics (MPs) has recently raised awareness surrounding the potential of human nanoplastics (NPs) exposure. Due to factors such as weathering, UV exposure, and other biodegradation processes, plastic pollution in the environment breaks down over time into micro (<5 micrometers) and nanoscale (<1000nm) particles....
On August 31, 1992, Quality Printing Circuits, a circuit board manufacturing plant in Phoenix, Arizona, burned to the ground. The fire lasted approximately eight hours, creating a thick, black smoke that blew into the surrounding community. Emergency evacuation was erratic and since no air samples were taken during the fire,...
This study investigates the applicability of the database-assisted design (DAD) methodology to predict structural reactions in a light-framed wood structure subjected to fluctuating wind pressures. Structural influence functions were determined on a 1/3-scale light-frame wood structure, which was then subjected to a wind flow, while the surface pressures and structural...
The Arc Marine data model is a generalized template to guide the implementation of geographic information systems (GIS) projects in the marine environment. Arc Marine developed out of a collaborative process involving research and industry shareholders in coastal and marine research. This template models and attempts to standardize common marine...