Neurofilament proteins are intermediate filaments found in the neuronal cytoskeleton. Phosphorylation of these proteins is considered important for the assembly and stability of the filaments. Accurate molecular weights have been difficult to measure, largely because the high degree of phosphorylation results in M[subscript r]'S that are significantly greater than dictated...
Hop cell wall material (CWM) extracted from hop leaves (hamulus lupulus) was
purified and characterized. The total sugar content, uronic acid content and
monosaccharide composition of the CWM were determined. Galacturonic acid is the
major component in the CWM. A mixture of unsaturated oligogalacturonides were
released from purified hop CWM...
Electron-biomolecule interactions are a biologically relevant field of study
because there are several ion fragmentation techniques that have come to the forefront
in mass spectrometric analysis that involve the interaction of charged peptides and
free electrons. By studying the electron energies that cause fragmentation in neutral
amino acids, amino acid...
E. coli thioredoxin (TRX) was modified by the episulfonium ion derived from S-(2-chloroethyl)glutathione (CEG) or S-(2-chloroethyl)cysteine (CEC). The alkylation site was located at Cys-32, which was confirmed by tandem mass spectrometry. Two forms of native TRX, Oxi- and Red-TRX, and two modified TRXs, GS- and Cys-TRX, were examined by hydrogen/deuterium...
Recombinant human macrophage colony stimulating factor beta (rhm-CSFβ) is a glycoprotein that stimulates the proliferation, differentiation and survival of cells belonging to the monocyte-macrophage lineage. It contains nine inter-subunit and intra-subunit disulfide bonds and represents an excellent model system for studying disulfide bond formation during protein folding because the assembly...
Resonance Electron Capture Mass Spectrometry (REC-MS) is a sensitive
experimental technique for the study of gas-phase compounds with positive electron
affinity. This technique was used to study a class of compounds known as
baloetbylenes containing chlorine and bromine substitutions. Haloetbylenes are an
important class of compounds because ethylene is the...
