A label-free, photoluminescence (PL) based biosensor for 2,4,6-trinitrotoluene (TNT) detection was developed by functionalizing diatom biosiilica with a TNT-specific single-chain variable fragment (scFv). The scFv loading was estimated to be 0.040±0.003 (μg scFv/μg biosilica). In saturated concentration, TNT binding to scFvbiosilica quenched about 13% of its PL. Dose response follows...
There is significant interest in utilizing microalgae as a source for biofuels. Diatoms are a class of single-celled microalgae which make silica cell walls and require dissolved silicon as a substrate for cell division. Manipulation of soluble silicon delivery to the culture offers a route to control cell cycle and...
Analytical chemistry is an essential qualitative and quantitative tool to describe foods and biomaterials and their changes during production, processing and distribution. In this work, rapid analytical procedures for the extraction and quantification of components in fluid milk and diatom fermentation broth were developed. Analytical methods to measure the concentration...