The formation of hydrogen peroxide (H₂O₂) by the FAD-dependent α-glycerophosphate oxidase (GlpO), is important for the pathogenesis of Streptococcus pneumoniae and Mycoplasma pneumoniae. The structurally known GlpO from Streptococcus sp. (SspGlpO) is similar to the pneumococcal protein (SpGlpO) and provides a guide for drug design against that target. However, M....
Mycoplasma pneumoniae is the primary cause of community-acquired pneumonias, including what is commonly known as walking pneumonia. This disease affects people from all demographics, but especially children and older adults. Outbreaks are a significant public health concern and work to develop new pharmacological agents is currently being researched. How M....
L-α-glycerophosphate oxidase is an FAD-dependent enzyme that catalyzes the oxidation of L-α-glycerophosphate (Glp) by molecular oxygen to generate dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H₂O₂). The catalytic properties of the recombinant His₆-GlpO from Mycoplasma pneumoniae (His₆-MpGlpO) were investigated with transient and steady-state kinetics and ligand binding. The results indicate that...
The function of a protein is defined by its three-dimensional structure, and in understanding the three-dimensional structure of a protein, we gain an understanding of its function and mechanism. Protein structures, especially at high resolution, can provide detailed insights into many elements of enzyme function and catalysis – identifying residues...
Oxygen binding behavior and subunit association-dissociation of
the hemocyanin from Callianassa are sensitive to several environmental
variables. Callianassa californiensis is a burrowing estuarine which
must survive changes in salinity, temperature and oxygen availability.
Previous work on the structure and function of the hemocyanin was
done under conditions which did not...
Sulfur is one of the six elements required during the early stages of the evolution of life, and enzymes involved in sulfur transfer and oxidation are increasingly being recognized as potential drug targets for antimicrobials as well as for therapies for cancer, neurodegenerative and inflammatory diseases. Bacteria are able to...
These short reports are intended to inform the public, industry, and other interested parties of the current studies of the Commission's staff and the basis for conservation measures. Reports will be published from time to time when studies are sufficiently complete to provide reliable biological evidence for conclusions upon which...
A simulation study of end-of-aisle order picking AS/R systems
is made with special emphasis on improving the system throughput by
introducing a zone near the home (Input/Output) location on each side
of the aisle, for storing partially picked pallets. The study considered
class-based-turnover assignment policies, one with a zone and...