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Filtering by: Subject Endothelial cells -- Cryopreservation

Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells

Creator:

Fry Davidson, Allyson

Abstract:

Cryopreservation of adherent cells may be advantageous for cell types that are difficult to preserve in suspension or when it is necessary to preserve characteristics of the adherent cultured cells. Vitrification is a promising procedure for the preservation of adherent cells that prevents ice crystal formation and the resulting dissociation...

Resource Type:

Dissertation

Full Text:

cell types and bodily fluids such as blood and lymph. Cryopreservation of endothelial cells is of
Removal of cryoprotectant with the use of a microseparation device

Creator:

Lusianti, Ratih E.

Abstract:

Cryoprotectants (CPAs) such as glycerol and dimethyl sulfoxide (DMSO) are commonly used during cryopreservation of cell based therapeutics. Although these additives are beneficial during freezing, it is often desirable to remove them before infusion into a patient. Currently, the most common method for CPA removal is by centrifugation. This method...

Resource Type:

Masters Thesis

Full Text:

during cryopreservation of cell based therapeutics. Although these additives are beneficial during
Improving the post-thaw processing of cryopreserved red blood cells using a combined approach of mathematical modeling and microfluidics

Creator:

Lusianti, Ratih E.

Abstract:

This study lays the groundwork on potential techniques that could be employed to improve the post-thaw wash processing of cryopreserved human red blood cells. Transfusion of red blood cells is one of the most commonly practiced procedures in clinical medicine. Millions of red blood cell units are transfused to patients...

Resource Type:

Dissertation

Full Text:

2.1 CRYOPRESERVATION OF RED BLOOD CELLS Cell based therapy can be defined as a form of medical