Five experiments were conducted to study the activity of the
pituitary gland in sheep as influenced by estrogen administered alone
or in combination with progesterone. Particular emphasis was given
to the study of gonadotropic activity.
Mature wethers (experiments I, III, IV and V) or ewes (experiment
II) were utilized as...
The binding of estrogen to endometrium and uterine arteries of the ewe was investigated by use of a [³H]estradiol exchange assay and by incubating whole tissue aliquants with [³ H]estradiol, a method which permits quantification of bound estrogen translocated from the cytoplasm to the nucleus. Five ewes were necropsied on...
The effects of estrogen treatment on uterine weight, uterine
nucleic acid content and adrenal weight were studied. Daily doses
of 0.05μg or 0.1 μg of Estradiol 17-β were administered subcutaneously
to seven day castrated female rats for periods varying
from 0 to 168 days. After two days of treatment uterine...
The effects of coumestrol on the uterine weight, uterine fluid
imbibition, uterine nucleic acid content, adrenal weight and body
weight of female rats were studied. Daily doses of 20 micrograms
of coumestrol per 0.1cc propylene glycol were administered subcutaneously
to spayed rats following a seven day recovery period,
for durations...
A total of five individual experiments were conducted with
gonadectomized, male sheep to study the influence of estrogen on
the plasma level of pituitary activities, with the greatest emphasis
given to gonadotropic activity. Estrogen treatments employed were
as follows:
1. 12 mg. diethylstilbestrol (DES) implanted subcutaneously.
2. 1 mg. DES...
Two experiments were conducted to determine whether in
ewes uterine concentrations of estrogen and progesterone
receptors are affected by the presence of a conceptus or by
the hormonal milieu associated with extremes in photoperiod
to which ewes are exposed.
In Exp.1, nine mature ewes were unilaterally
ovariectomized by removing an...