Excess fatty acids and sterols are stored as triacylglycerols and sterol esters in
specialized cellular organelles, called lipid droplets. Understanding what determines the cellular
amount of neutral lipids and their packaging into lipid droplets is of fundamental and
applied interest. Using two species of fission yeast, we show that cycling...
Gene regulatory networks control the state of a cell. Models of such networks have been produced for sea urchin development and suggest that cell type may be defined by distinct network kernels. Such kernels are composed of a set of sequence specific DNA-binding transcription factors (SSTFs) that maintain each other’s...
It has been shown that about 50 % of the
whole chromatin DNA exists in a very compact form in calfthymus
chromatin.These compact fragments can be extracted
from the whole chromatin as nuclease resistant fragments
by limited nuclease digestion. These fragments involve four
out of five histones in folding the...
WaRSwap is a randomization algorithm that for the first time provides a practical network motif discovery method for large multi-layer networks, for example those that include transcription factors, microRNAs, and non-regulatory protein coding genes. The algorithm is applicable to systems with tens of thousands of genes, while accounting for critical...
The body of work aims to contribute to the area of Quantitative analysis in Environmental Sciences, and is focused on the microbiome. The underlying theme for the dissertation is methods and workflow. The unique bioinformatics workflow used for the analysis of genomic data was used to answer distinct and pressing...
Kinetics of cell division in the intestinal epithelial proliferative cells (cell nests) of the rough-skinned newt, Taricha granulosa, were studied using tritiated thymidine autoradiography and the mitotic arresting properties of colcemid. Percent labeled mitoses (PLM) curves were drawn from the autoradiographic data from two separate experiments in which the newts...
The overall focus of this thesis is on the distribution of specific lipids and membrane proteins of the external and internal membranes of plant cells, in the context of the roles that those lipids and proteins may play in microbe-plant interactions. The work includes the development of several new tools,...