We previously reported that inhibition of the Na⁺ translocating NADH:ubiquinone oxidoreductase (NQR), either by chemical inhibition or mutation, increased toxT transcription in Vibrio cholerae. In this study, we revealed that the nqr mutant strain showed similar phenotypes as the Escherichia coli NADH dehydrogenase I (nuo) mutant strain (e.g. growth defect...
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% Urea
Mode of action
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Sara R. Fassio1, Yusuke Minato2, Matthew J. Quinn1, WyattJ. Faulkner1
We previously reported that inhibition of the Na⁺ translocating NADH:ubiquinone oxidoreductase (NQR), either by chemical inhibition or mutation, increased toxT transcription in Vibrio cholerae. In this study, we revealed that the nqr mutant strain showed similar phenotypes as the Escherichia coli NADH dehydrogenase I (nuo) mutant strain (e.g. growth defect...
Full Text:
Mode of action Substrates
Sara R. Fassio1, Yusuke Minato2, Matthew J. Quinn1, WyattJ. Faulkner1 and
We previously reported that inhibition of the Na⁺ translocating NADH:ubiquinone oxidoreductase (NQR), either by chemical inhibition or mutation, increased toxT transcription in Vibrio cholerae. In this study, we revealed that the nqr mutant strain showed similar phenotypes as the Escherichia coli NADH dehydrogenase I (nuo) mutant strain (e.g. growth defect...
The objective of this study was to determine the effects of processing on sodium - potassium and calcium - phosphorus ratios in tuna canned in oil and in water, peanut butter, white and whole wheat flours. Mineral levels were determined by atomic absorption spectrophotometry in food samples at various stages...
Absorption of iron from foods has been reported to be inhibited by
various factors including some diet components. Fiber from different
sources has been observed to bind ferrous iron. This may be the reason
for iron deficiencies observed in populations that consume diets high in
non-digestible materials.
The effect of...
Current retail food distribution practices, microbial quality of
bakery products, and the potential for growth of food borne pathogens
(Staphylococcus aureus, Salmonella typhimurium, and Bacillus cereus)
were examined using pumpkin pie as a model.
The pumpkin pies, as obtained from the retail outlets, showed contamination
with food pathogens. The aerobic...
The objective of this project was to evaluate various culturing
and isolation techniques of Campylobacter jejuni and to develop methods
to detect the organism in foods. The morphological, cultural and biochemical
characteristics of C. jejuni were studied using developed
microbiological methods. A variety of media, broths, microaerophilic
atmospheres and diluents,...
Glycomacropeptide (GMP) is a bioactive peptide found in the dairy product whey, along with whey protein isolate (WPI). Past studies have shown that digested GMP retains bioactivity in rodent guts. This study tested whether GMP or GMP-derived peptides or whey protein or whey protein-derived peptides inhibit pro-inflammatory cytokines. THP-1 cells...
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Wyatt Olsen, Committee Member, representing College of Public Health & Human Sciences
Na⁺/H⁺ antiporters are ubiquitous membrane proteins that play a central role in the ion homeostasis of cells. In this study, we examined the possible role of Na⁺/H⁺ antiport in Yersinia pestis virulence and found that Y. pestis strains lacking the major Na⁺/H⁺ antiporters, NhaA and NhaB, are completely attenuated in...
The Na⁺ translocating NADH:quinone oxidoreductase (Na⁺-NQR) is a unique respiratory enzyme catalyzing the electron
transfer from NADH to quinone coupled with the translocation of sodium ions across the membrane. Typically, Vibrio spp.,
including Vibrio cholerae, have this enzyme but lack the proton-pumping NADH:ubiquinone oxidoreductase (Complex I).
Thus, Na⁺-NQR should significantly...
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Yusuke Minato1¤, Sara R. Fassio2., Jay S. Kirkwood3,4., Petra Halang5, Matthew J. Quinn2,
WyattJ