Fluorescence of adenine, guanine, thymine, cytosine, and uracil
has been detected at room temperature in neutral aqueous solution
using a digital signal accumulation technique and right angle fluorescence
detection from solutions with absorbances between 0. 4 and 0. 7.
The quantum yields are respectively 2.6 x 10⁻⁴, 3.0 x 10⁻⁴,...
An automated multielement flame atomic fluorescence (AF) spectrometer based on microcomputer control was constructed to determine four elements simultaneously. The instrument employs a multiple exit slit monochromator where the light from various exit slits is directed to a single detector with a mirrored funnel. Each element is excited to fluoresce...
Time-resolved fluorescence spectroscopy was used to monitor the effects of
varying ionic strength on nucleosome core particle structure. Two main methods were
used in these studies. First, the fluorescence anisotropy decay of bound ethidium was
measured and was shown to reflect the rotational tumbling of the core particle through
solution,...
An apparatus and method for calibrating planar laser induced fluorescence using a two-dye, two-color method was developed. A laser sheet was expanded from a 473 nm laser to excite dye solutions into higher energy states then fluoresced through spontaneous emission. The two fluorescent dyes selected for research and development were...
Coral reefs have become extremely vulnerable to rising ocean temperatures, with mass bleaching events increasing in frequency and severity. If bleaching events persist annually, models predict that more than 90% of reef species will face long-term degradation. However, recent evidence has shown that corals may be able to thermally acclimate...
Research was conducted to determine the distance and amount
of outcrossing between varieties of highly fluorescent annual ryegrass
(Lolium multiflorum Lam.) and low-fluorescing perennial
ryegrass (Lolium perenne L.). Studies were conducted under both
spaced-plant and seed production field conditions.
Fluorescence tests on seed collected from low-fluorescing
perennial ryegrass at successive...
The influence of nitrate availability and irradiance on phytoplankton natural fluorescence was investigated in laboratory cultures of the marine diatom Thalassiosira weissflogii (Bacillariophyceae). Two cultures of phytoplankton, differing only in the nitrate-limited growth rate, were compared to learn how increases in irradiance influences natural fluorescence. Instrumentation was developed for these...