Self-assembly of recombinant proteins within the biosilica of living diatoms represents a means to construct functional materials in a reproducible and scalable manner that will enable applications that harness the inherent specificities of proteins to sense and respond to environmental cues. Here we describe the use of a silaffin-derived lysine-rich...
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Xiong,† NicoleR. Ford,‡ Karen A. Hecht,‡ Guritno Roesijadi,‡, § and
Thomas C. Squier
Self-assembly of recombinant proteins within the biosilica of living diatoms represents a means to construct functional materials in a reproducible and scalable manner that will enable applications that harness the inherent specificities of proteins to sense and respond to environmental cues. Here we describe the use of a silaffin-derived lysine-rich...
Self-assembly of recombinant proteins within the biosilica of living diatoms represents a means to construct functional materials in a reproducible and scalable manner that will enable applications that harness the inherent specificities of proteins to sense and respond to environmental cues. Here we describe the use of a silaffin-derived lysine-rich...
The diatom Thalassiosira pseudonana was genetically modified to express biosilica-targeted fusion proteins comprising either enhanced green fluorescent protein (EGFP) or single chain antibodies engineered with a tetracysteine tagging sequence. Of interest were the site specific binding of (1) the fluorescent biarsenical probe AsCy3 and AsCy3e to the tetracysteine tagged fusion...
The present study examined involuntary attentional bias toward facial emotion expression. Particularly, the study examined two different attentional components for emotional processing, namely, orienting vs. disengagement. A cueing paradigm using two cue presentation times (250ms and 350ms) was used to determine if attention would be involuntarily captured by an irrelevant...
A selective and label-free biosensor for detection of the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solution was developed based on the principle of photoluminescence quenching of upon immunocomplex formation with antibody-functionalized diatom frustule biosilica. The diatom frustule is an intricately nanostructured, highly porous biogenic silica material derived from the shells...
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an anti-TNT monoclonal antibody fragment
Le Zhena, Nicole Fordb, Debra K. Galea, Guritno
A selective and label-free biosensor for detection of the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solution was developed based on the principle of photoluminescence quenching of upon immunocomplex formation with antibody-functionalized diatom frustule biosilica. The diatom frustule is an intricately nanostructured, highly porous biogenic silica material derived from the shells...
Full Text:
biosilica functionalized with an anti-TNT monoclonal
antibody fragment
Le Zhen a, NicoleFord b, Debra K
A selective and label-free biosensor for detection of the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solution was developed based on the principle of photoluminescence quenching of upon immunocomplex formation with antibody-functionalized diatom frustule biosilica. The diatom frustule is an intricately nanostructured, highly porous biogenic silica material derived from the shells...
Large numbers of hatchery salmon spawn in wild populations each year. Hatchery
fish with multiple generations of hatchery ancestry often have heritably lower
reproductive success than wild fish and may reduce the fitness of an entire population.
Whether this reduced fitness also occurs for hatchery fish created with
local- and...
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; Ford et al. 2013). Males that never went out to sea (or at least those that did not pass below the
Large numbers of hatchery salmon spawn in wild populations each year. Hatchery
fish with multiple generations of hatchery ancestry often have heritably lower
reproductive success than wild fish and may reduce the fitness of an entire population.
Whether this reduced fitness also occurs for hatchery fish created with
local- and...
Full Text:
Christie, M. R., Ford, M. J. and Blouin, M. S. (2014). On the reproductive success
of early-generation