Procedures were developed for assaying the biosynthesis of (-)-
kaurene-¹⁴C and other intermediates in gibberellin (GA) synthesis
from mevalonic acid-¹⁴C in cell-free enzyme extracts of immature
pea (Pisum sativum L. cv. Alaska) seeds. This system was utilized
to investigate three aspects of GA biosynthesis: (1) apparent capacities
for (-)-kaurene biosynthesis...
Previous investigations have shown that the enzymes
catalyzing the biosynthesis of the gibberellin-type of hormones
from mevalonic acid in plants are compartmentalized
in plastids. Moreover, the activity of one of the key
enzymes in the biosynthetic sequence, kaurene synthetase,
exhibits markedly enhanced activity when a dark-grown pea
seedling undergoes de-etiolation...
An understanding of the gibberellins present in Douglas-fir is essential to understanding and controlling the growth and development
of this species. A technique was developed which permits the separation of certain gibberellins with high performance liquid chromatography and their identification with mass spectrometry. An extraction technique
for the isolation of...
Experiments were performed to determine whether a direct
correlation exists between the growth rates of tall and dwarf peas
grown either in the light or in the dark and endogenous gibberellin
(GA) content. Three separate but related experimental approaches
were utilized: (1) the determination of possible qualitative and /or
quantitative...
