Contact of blood with the surfaces of synthetic materials is associated with spontaneous protein adsorption, initiating platelet aggregation, the coagulation cascade, and the eventual development of a stable clot. Current therapy to inhibit implant-induced thrombosis is life-long administration of systemic anticoagulants. An alternative to the systemic administration of anticoagulant drugs...
As a major natural component of the extracellular matrix (ECM),
hyaluronic acid (HA) is an excellent choice for biomimetic, biocompatible
therapeutic materials. Furthermore, thiol-modified forms of HA are capable of
forming macroporous hydrogels that allow for both controlled cytokine release and
extensive vessel in-growth. It has previously been shown that...
Biocompatible hydrogels composed of covalently crosslinked, chemically modified hyaluronan (HA), gelatin (Gtn), and heparin (Hp) were synthesized for controlled release of human vascular endothelial growth factor (VEGF) or basic fibroblast growth factor (bFGF). We hypothesized that inclusion of small quantities of heparin in these gels would result in regulated growth...
Heparin was modified with adipic diliydrazide and covalently linked to surface-activated silica. Contact angle measurements were made to determine changes in surface at various stages of the derivatization. Xray photoelectron spectroscopy was used to analyze the elemental composition of the surface at each step of immobilizing heparin as well and...
Heparin was modified and immobilized to surface-activated silica surfaces using two different reaction schemes. End-aminated heparin was reacted with 2-iminothiolane to produce free thiol groups at the terminal ends of the heparin chains. The end-thiolated heparin was immobilized by reaction with a pyridyl disulfide activated poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] triblock...
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Joseph McGuire
Heparin was modified and immobilized to surface-activated silica surfaces
Nisin, an antibacterial peptide proven to be an effective inhibitor of Gram-positive bacteria, was incorporated into novel block copolymer constructs and tested for retained antibacterial activity. Covalent coupling was achieved by chemical modification of the N-terminal isoleucine to introduce a thiol group. Thiolated nisin derivatives were then linked to poly[ethylene...
Adipic dihydrazide-modified heparin was prepared and
subsequently immobilized on surface-activated silica wafers. X-ray
photoelectron spectroscopy (XPS) was used to quantify the elemental
composition of the silica surface at each stage of activation and results
showed that successful immobilization had taken place. Contact angle
measurements were made to verify the high...