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  • Structural and functional dissection of the vaccinia virus thymidine kinase enzyme

    Creator:
    Black, Margaret E.
    Abstract:
    Thymidine kinase is a key enzyme in the nucleotide salvage pathway, catalyzing the production of dTMP from thymidine and ATP. In order to identify the structural features of the TK protein and/or primary amino acid sequences which contribute to the catalytic and regulatory activities of this enzyme, an in vitro...
    Resource Type:
    Dissertation
    Full Text:
    Vaccinia Virus Thymidine Kinase Enzyme Abstract approved: Redacted for Privacy Dr. Dennis E. Hruby

  • Replication of vaccinia virus in the presence of 1,10-phenanthroline

    Creator:
    ApRoberts, Robyn E.
    Abstract:
    1,10-phenanthroline and its non-chelating isomer, 1,7-phenanthroline were used to inhibit the replication of vaccinia virus (VV). Serial passage of VV in the presence of various concentrations of either 1,10-phenanthroline or 1,7-phenanthroline was carried out. No drug resistant mutants were isolated, suggesting that the observed inhibition was due to a cellular...
    Resource Type:
    Masters Thesis
    Full Text:
    1,10-phenanthroline. Abstract approved: Dennis E. Hruby 1,10-phenanthroline and its non

  • Molecular genetic analysis of vaccinia virus genes which confer resistance to alpha-amanitin

    Creator:
    Tamin, Azaibi
    Abstract:
    Several laboratories have shown that the VV replication cycle is dependent on the host cell nucleus. The basic biological observation is that if the host cell nucleus is physically removed by cytochalasin B-mediated enucleation, functionally inactivated by UV-irradiation, or selectively blocked with a-amanitin, the results are the same: W is...
    Resource Type:
    Dissertation
    Full Text:
    Dr. Dennis E. Hruby Several laboratories have shown that the VV replication cycle is dependent on

  • The regulation of a vaccinia virus late gene required for morphogenesis

    Creator:
    Miner, Jeffery N.
    Abstract:
    Vaccinia virus gene expression is characterized by tight temporal regulation. Viral early transcription begins within the cytoplasm upon infection; three hours later, early genes are repressed, DNA replication occurs and late gene expression commences. As an approach toward understanding the mechanisms governing vaccinia late gene expression, I have undertaken an...
    Resource Type:
    Dissertation
    Full Text:
    Morphogenesis Abstract approved: Redacted for Priv/ ac)/ Dr. Dennis E. Hruby Vaccinia virus gene expression is

  • Advances in expression of heterologous protein products by Streptococcus gordonii

    Creator:
    Warren, Travis Kent
    Abstract:
    Streptococcus gordonii is a bacterial species that naturally colonizes the oral cavity of most healthy humans. It resides in the mouth as an adherent to dental surfaces and, with few exceptions, does not cause disease in individuals it inhabits. It possesses qualities that encourage its use as a vector to...
    Resource Type:
    Dissertation
    Full Text:
    : ______________________________________ Dr. Dennis E. Hruby Streptococcus gordonii is a bacterial species that naturally colonizes

  • Structure-function analysis of the vaccinia virus I7L proteinase

    Creator:
    Moerdyk, Megan J.
    Abstract:
    Vaccinia virus is the prototypic member of the Orthopoxvirus genus. It undergoes a complex replication process where a key step in the transition from immature virion to intracellular mature virion is the cleavage of the major core protein precursors. The product of the I7L open reading frame (ORF) is a...
    Resource Type:
    Masters Thesis
    Full Text:
    approved: ______________________________________________________________ Dennis E. Hruby

  • Biology of a constitutively-expressed vaccinia virus gene required for DNA replication

    Creator:
    Roseman, Nancy A.
    Abstract:
    Replication of vaccinia virus in the cytoplasm of the infected cell occurs under the direction of virally encoded gene products. Expression of approximately 200 viral genes follows a highly regulated temporal scheme which can be followed biochemically and morphologically during the assembly process. In order to dissect this complex genetic...
    Resource Type:
    Dissertation
    Full Text:
    friendship and some aspects of sanity. Ultimately, I have to thank Dr. Dennis E. Hruby for financial

  • Host cell nuclear involvement in vaccinia virus replication

    Creator:
    Villarreal, Elcira C.
    Abstract:
    It was previously shown that vaccinia virus is unable to complete its replicative cycle in cells that have been subjected to cytochalasin-B mediated enucleation prior to infection. Likewise, in the presence of the drug α-amanitin, a potent inhibitor of host RNA polymerase II but not of viral transcription, the replication...
    Resource Type:
    Dissertation
    Full Text:
    like to extend my appreciation to Dr. Dennis E. Hruby for arranging my personal financial support

  • Investigation of the roles of viral proteinases in the vaccinia virus life cycle

    Creator:
    Page, Jessica M.
    Abstract:
    An inducible, mutant virus, designated vvtetO:I7L/G1L, was used to study the morphogenic proteolysis step of the vaccinia virus life cycle. The vvtetO:I7L/G1L controlled the expression of two genes, I7L, a cysteine proteinase, and G1L, a putative metalloproteinase. These proteins are involved in the maturation of viral core proteins, p4a, p4b,...
    Resource Type:
    Honors College Thesis
    Full Text:
    Dennis Hruby An inducible, mutant virus, designated vvtetO:I7L/G1L, was used to study the morphogenic

  • Proteolytic maturation of vaccinia virus structural proteins

    Creator:
    VanSlyke, Judy K.
    Abstract:
    Vaccinia virus (VV) is a large DNA virus belonging to the Orthopoxvirus family. The viral replicative life cycle takes place solely within the cytoplasm of a mammalian host cell. The VV genome contains 196 open reading frames which are expressed in a highly regulated and temporal fashion in order to...
    Resource Type:
    Dissertation
    Full Text:
    Proteins Abstract approved: Dr. Dennis E. Hruby Vaccinia virus (VV) is a large DNA virus belonging to