The objective of this study was to examine optimum conditions for primary
culture of porcine preadipocytes. Dorsal subcutaneous adipose tissue was obtained
from biopsies of 40-50 kg pigs and digested with collagenase in Krebs Ringer
Bicarbonate buffer. Isolated stromal vascular cells were cultured in different test media.
Stromal vascular cells...
Three experiments were conducted (1) to develop and characterize
polyclonal antibodies against ovine adipocyte plasma membranes (APM),
(2) to evaluate the effect of administering the developed antibodies to
lambs on lamb growth and carcass characteristics, and (3) to investigate
the possibility of using the developed antibodies to identify antigenic
The accumulation of excessive fat is a serious concern in both
the livestock production and human health fields. Obesity is a
condition of excessive energy storage in the form of body fat
( triacylglycerols ). The cellular basis for obesity is not yet
understood but numerous factors have been suggested....
Primary cultures of stromal-vascular (S-V) cells from adipose tissue were
used to investigate the regulation of preadipocyte development. Differentiation of
S-V cells was found to be under hormonal control. Insulin and glucocorticoids are
essential for S-V cell differentiation in culture.
S-V cells from both newborn and mature pig adipose tissue...
In recent years, the general public has recognized the dangers of a high fat diet and
are demanding meat with lower fat content. This demand has stimulated research in the
growth and regulation of adipocytes. However, despite much effort, no adipocyte-specific
plasma membrane markers from any species are available as...
The incidence of obesity has increased in the U.S. until an obesity epidemic now exists. American consumers are acutely aware of the consequences of obesity and are increasingly associating the consumption of high-fat foods with having a negative impact upon their health. This has in turn forced producers to grow...
Evidence suggests that adipose tissue is not a homogeneous entity,
but rather that it differs functionally and morphologically according to its
anatomical location. Production of a site-specific monoclonal antibody
(MAb) is therefore of interest, not only for the study of adipocyte
development and differentiation, but also for the elimination of...