Salmonid B lymphocytes were stimulated both in vivo and in vitro
and shown to produce antibody specific for the immunogen. A passive
hemolytic plaque assay was employed to demonstrate B cells producing
specific antibody. Characterization of plaque forming cells (PFCs)
involved sequential inhibition with a series of graded concentrations
of...
Renibacterium salmoninarum produces bacterial kidney
disease (BKD) in salmonid fish. Soluble antigen(s) (SA) produced
by the bacterium was characterized by physicochemical,
functional and immunological methods. The molecular weight
determination by SDS-PAGE analysis of SA yielded 14 different
molecular weight species. Seven of these species were
prominent, 57 kd protein being...
The in vitro effects of cortisol in regulating salmonid B cell functions were
investigated. B cells at three distinct stages of differentiation were examined for
cortisol sensitivity. B lymphocyte responses were examined 1) during the early stage
of the precursor, 2) during the intermediate stage of differentiation associated with
clonal...
The foundation of the research compilation presented here began
with the derivation of an improved method for obtaining optimal in vitro
mitogenic responses in salmonid lymphocyte cultures by utilizing
autologous or homologous plasma as the primary serum supplement. It was
observed that lymphocytes which were previously unresponsive to
mitogenic challenge...
Little is known about the virulence factors of Renibacterium
salmoninarum, the causative agent of salmonid bacterial kidney disease.
The predominant protein produced by R. salmoninarum in broth culture or
during infection is a 57/58 kDa protein (p57) which is associated with strain
virulence. In this study monoclonal antibodies (MAbs) to...
Currently little is known about the mechanisms and locations of
lymphocyte development in teleosts. In this study several aspects of the
underlying factors which govern B lymphocyte development in trout
were investigated which included: the isolation and characterization of
immunoglobulin heavy chain (IgH) genes, the recombination activating
genes 1 and...
Short non-flexible elements of cable were driven harmonically
in water while constrained by springs. The cable elements were observed
to oscillate at approximately the Strouhal vortex shedding
frequency. Damped resonance occurred near the point where the natural
frequency and the Strouhal vertex shedding frequency were equal.
Transverse oscillation amplitudes greater...
Optimization of reservoir operation is involves various competing objectives for a scarce resource (water). To find the optimal operation of reservoirs, it is essential to consider multiple objectives simultaneously. There are various sources of uncertainty associated with the reservoir operation problem that should be considered as well.
The overarching goal...