Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in
which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity.
Thus, we investigated the use of physics-based mathematical optimization to guide design of
cryoprotectant loading methods for mouse and human oocytes. We first...
Studies were directed toward the formulation of a selective
Aeromonas medium that would permit the isolation of Aeromonas
species in the presence of other common bacterial flora. The final
composition of this medium, PBG Agar, in grams per liter, is:
Bacto-peptone, 10; Bacto-beef extract, 10; glycogen, 4; NaCl, 5;
sodium...
Marine systems undergo changes in community composition over time as a result of a variety of environmental and anthropogenic factors. Understanding these community changes and the factors that drive them is critical for ecosystem management of marine resources. The Gulf of Alaska (GOA) is a large marine ecosystem that includes...
Six experiments were carried out with Single Comb White Leghorn laying chickens to
assess the effect of feeding a source of direct-fed microbials (Lactobacillus; Lacto) and its
carrier [condensed cane molasses solubles (CCMS)] on the retentions of fat, nitrogen and
several minerals; on the status of the pH of the...
The implementation of advanced hybrid (Monte Carlo/Deterministic) transport methods for realistic test problems has been a challenge due to the overhead efforts associated with interfacing a solution generated by a deterministic solver with a Monte Carlo based radiation transport code. In this work, with the help of Transpire, Inc., a...
The natural production of methane via anaerobic digestion (AD) is facilitated by a complex community of microorganisms, primarily bacteria and archaea, referred to as the AD microbiome. AD is growing in prominence as a solution to the disposal of food waste, sewage sludge, and the organic fraction of municipal solid...
Ethanol production from lignocellulosic feedstock has been under intense scrutiny as a transportation fuel due to its potential to address concerns of increasing energy consumption, limited fossil energy resources, climate changes due to greenhouse gas emissions from fossil fuels, and especially use of non-food biomaterials, which address the biggest limitation...
Radiation exposure to the brain can occur from a variety of events, including space missions, nuclear accidents, and clinical treatments. The effects of high dose radiation are well studied, but those of lower dosage are more elusive. Decline of memory and cognition is observed in patients exposed to low dose...
Food waste (FW), brewery waste (BW), and fats, oils, and greases (FOG), are prevalent waste streams in municipalities across the country, particularly in the Pacific Northwest (PNW). Anaerobic digestion (AD), the microbial-mediated transformation of organic waste into methane-rich biogas, offers a sustainable, energy-generating, waste management solution for these wastes. Anaerobic...