In this five-week feeding study male New Zealand rabbits were
fed diets containing cyclopropene fatty acids (CPFA), diets containing
cholesterol, and diets with both CPFA and cholesterol added to test
the effect of CPFA on cholesterol metabolism and atherosclerosis
induction. When CPFA-fed animals were compared with control
animals, they tended...
Micro-organisms associated with gari, produced in Cameroun,
Africa, by the traditional method and that produced in the laboratory,
were isolated, identified, and screened for their abilities to produce
gari flavor. Gari production, under the controlled conditions was
attempted utilizing these isolates and the dairy starter cultures.
Some of the volatiles...
The cyclopropene fatty acids, sterculic and malvalic, occur
naturally in a major food lipid, cottonseed oil, and have been implicated
in several physiological disorders. Rainbow trout in this
study were fed semipurified diets containing 200 or 300 ppm methyl
sterculate, and the effects on hepatic lipid and glycogen levels,
lipid...
Aflatoxins B₁, Q₁ and aflatoxicol, R [subscript o] F, but not B₂ were activated in the presence of NADPH by the 105,000 x g pellet from rainbow
trout (Salmo gairdneri), Mt. Shasta strain, liver to products lethal to
Bacillus subtilis GSY 1057 (metB4, hisAl, uvr-1). Electron microscopy
confirmed the microsomal...
Interactions between paralytic shellfish poison (PSP) and
components of the butter clam (Saxidomus giganteus) were studied to
determine which component was involved in the binding and retention
of PSP by the butter clam, and to characterize the nature and
strength of the interaction. A simple and accurate assay for PSP...
The microbial composition of Pacific oysters (Crassostrea gigas)
subjected to UV-treated seawater was determined by quantitative and qualitative means. A total of 2,779 microorganisms were identified from seawater and oysters during a 72 hour sampling period employing a computer assisted replica-plating technique. UV treatment effectively eliminated coliforms and Pseudomonas Type...
Some kinetics of the oxygen requirement of amine oxidases
have been investigated using bovine plasma monoamine oxidase (PAO)
and pea seedling diamine oxidase (DAO). A system is described for
studying enzyme reactions under controlled oxygen tension and in an
O₂-free N₂ atmosphere.
O₂ Km values were determined at two different...
The design and construction of a chemiluminescence
photometer is described. The design is based on a discrete
sample system which has a number of advantages over
the previously described flow cell system and centrifugal
analyzer. The advantages include simple and inexpensive
equipment, small sample size, versatile electronic readout
system, and...
The application of lucigenin chemiluminescence for the determination
of trace concentrations of Co(II) is described. This method
is based on the enhancing effect that Co(II) has on the reaction of
lucigenin and hydrogen peroxide in a basic solution. The chemiluminescence
signal from a Co(II) enhanced lucigenin reaction, which is in...