The A segment of infectious pancreatic necrosis virus (IPNV)
is expressed as a polyprotein encoding three primary gene
products, VP2, NS and VP3, from a large open reading frame. The
nucleotide sequence for the A segment of the Sp isolate of IPNV
was determined. The NS protein is the putative...
Hatchery-reared salmonid fish routinely encounter stress due to
handling, barging, tagging, and overcrowding. It has been demonstrated that
there exists a direct correlation between stress and transient immune
suppression which can last for many days in fish. Epizootic viral infections
routinely appear in hatcheries and can have a devastating effect...
Molecular biological approaches were used to study and interfere with the life
cycle of infectious hematopoietic necrosis virus (IHNV). These included the control
of IHN disease in rainbow trout by genetic immunization or interference in vitro by
synthesis of sense and antisense expression of the viral nucleocapsid (N) gene, and...
The role of viral proteins in the pathogenesis of infectious hematopoietic necrosis
virus (IHNV) was studied at the molecular level. The expression of the viral genes at the
protein and RNA level, and their cellular localization, were characterized to further our
understanding of viral pathogenesis. The pathogenic effect of individual...
The Mx protein family of rainbow trout (Oncorhynchus mykiss) was characterized at the molecular level. With primers derived from a partial sequence of Perch (Perca fluviatis) Mx genomic DNA, a partial rainbow trout Mx genomic DNA sequence was obtained by polymerase chain reaction (PCR) amplification. All salmonid fish investigated contained...
The complete genome of snakehead rhabdovirus (SHRV) was cloned and
molecularly characterized. This was initially accomplished through the sequence
determination of its glycoprotein gene and the phylogenetic analysis of this gene with
orthologous genes from other rhabdoviruses. The phylogenetic analysis revealed that
SHRV groups with viruses of the genus Novirhabdovirus....
Recent advances in DNA vaccine technology has brought about a promising strategy for the control of viruses that contain surface membrane glycoproteins. This type of vaccine involves the intramuscular injection of a bacterial plasmid containing a gene encoding a viral protein. The strategy uses eukaryotic processing of the protein as...