The use of consultation as a means of delivering educational
instruction to students with disabilities in the general physical education
setting is becoming increasingly prevalent in the United States and is most
frequently operationalized in a triadic model. In this model the adapted
physical educator serves as the consultant, the...
Understanding of hydroclimatic processes in Africa has been hindered by the lack of in situ precipitation measurements. Satellite-based observations, in particular, the TRMM Multisatellite Precipitation Analysis (TMPA) have been pivotal to filling this void. The recently released Integrated Multisatellite Retrievals for GPM (IMERG) project aims to continue the legacy of...
The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a
long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation
of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Full Text:
Fluorescent Amino Acid for Lifetime and
FRET/LRET Studies
Lee C. Speight,† Anand K. Muthusamy,† Jacob M
The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a
long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation
of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Full Text:
FRET/LRET Studies
Lee C. Speight,† Anand K. Muthusamy,† Jacob M. Goldberg,† John B. Warner,† Rebecca
The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a
long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation
of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Next-generation DNA sequencing (NGS) approaches are rapidly surpassing Sanger sequencing for characterizing the diversity of
natural microbial communities. Despite this rapid transition, few comparisons exist between Sanger sequences and the generally
much shorter reads of NGS. Operational taxonomic units (OTUs) derived from full-length (Sanger sequencing) and pyrotag (454
sequencing of...
Rising atmospheric [CO₂], cₐ, is expected to affect stomatal regulation of leaf gas-exchange of woody plants, thus influencing energy fluxes as well as carbon (C), water, and nutrient cycling of forests. Researchers have proposed various strategies for stomatal regulation of leaf gas-exchange that include maintaining a constant leaf internal [CO₂],...