The genus Bdellovibrio is characterized by the unique ability to
parasitize and enter gram-negative host bacteria. This interaction
leads to the consumption and death of the host cell as the Bdellovibrio
grows and divides into progeny. Little is known about the metabolism
of host cell components by Bdellovibrio. The possibility...
A microfluidic nanofiltration module has been designed, fabricated and applied to the continuous-flow, pressure-driven, post-synthetic purification of macromolecules and nanoparticles via diafiltration using a commercially available organic solvent resistant nanofiltration membrane, STARMEM 122. This module will readily interface with other microscale components within a
"nanofactory" for the rapid synthesis, purification...
The halogen bond is a non-covalent, stabilizing interaction analogous to a hydrogen bond in which an anisotropically polarized halogen atom interacts
electrostatically with a Lewis base. Until very recently, the ability of halogens to form these stabilizing interactions in biological macromolecules was all but unknown, but
examples of halogen bonding...
Methods are presented to acquire data from analytical ultracentrifugation experiments
by computer using the absorption optical scanning system of the Beckman Model-E
ultracentrifuge. A computer program was written which analyzes sedimentation velocity
experiments by the van Holde - Weischet method and by the second moment method. The van
Holde -...
A variety of macromolecules labeled with FITC (fluorescein isothiocyanate), including
carbohydrates (dextran) and proteins (ferritin, casein, albumin, concavalin A), spanning a range of
molecular weights from 55 to 2000 kD, were actively ingested by marine heterotrophic flagellates at
substrate concentrations of 1 to 10 mg/L. The uptake capacity for the...
Crystallographic studies of ligands bound to biological macromolecules (proteins and nucleic acids) represent an important source of information concerning drug-target interactions, providing atomic level insights into the physical chemistry of complex formation between macromolecules and ligands. Of the more than 115,000 entries extant in the Protein Data Bank (PDB) archive,...
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Crystallographic studies of ligands bound to biological macromolecules (proteins and
Crystallographic studies of ligands bound to biological macromolecules (proteins and nucleic acids) represent an important source of information concerning drug-target interactions, providing atomic level insights into the physical chemistry of complex formation between macromolecules and ligands. Of the more than 115,000 entries extant in the Protein Data Bank (PDB) archive,...
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descriptions of constituent molecules in experimentally
determined 3D macromolecules in the Protein Data Bank
Crystallographic studies of ligands bound to biological macromolecules (proteins and nucleic acids) represent an important source of information concerning drug-target interactions, providing atomic level insights into the physical chemistry of complex formation between macromolecules and ligands. Of the more than 115,000 entries extant in the Protein Data Bank (PDB) archive,...
The marine psychrophilic bacterium Vibrio marinus MP-4
possessed a maximum temperature and hydrostatic pressure of
20 C and 425 atm for growth. The effects of temperatures of 21 and
25 C and hydrostatic pressures of 200, 400, 500, and 1,000 atm on
protein, RNA and DNA synthesis by V. marinus...