Three newborn, colostrum deprived calves were inoculated by the intranasal, intratracheal, conjunctival and oral routes with a subgroup II bovine adenovirus strain 7T. An initial viremia and febrile response occurred in all calves with widespread dissemination of the virus through the body. Clinical signs consisted of pyrexia, soft feces and...
In October 1977, a study was undertaken to determine the role of bovine adenovirus type 3 in enzootic pneumonia of calves, and the efficacy of using a vaccine for prevention of disease. Bovine adenovirus type 3 was isolated from the herd under study in the previous year. Calves in the...
Four bovine adenovirus type 3 isolates, recovered from different areas in Oregon and Washington, were compared antigenically with the original prototype strain which was isolated in England. The fluorescent foci assay method was used to quantitatively establish viral titers. A serum-viral neutralization test was performed with a standard dose of...
One hundred and fourty-one lambs showing signs of pneumonia
and/or enteritis from four different flocks in western Oregon were
sampled for virus isolation. Two ovine adenoviruses, antigenically
distinct from each other and from bovine adenoviruses, were
isolated from the nasal mucosa and feces of two lambs. The lambs
were showing...
A kinetics-based enzyme-linked immunosorbent assay (KELA) for detection of serum IgG antibody against bovine respiratory syncytial virus (BRSV) was developed and optimized. Optimization factors tested included brand of microELISA plate, length of incubation periods at various temperatures, type of conjugate, purified versus precipitated antigen preparations, type of dilution buffer as...
An enzyme-linked immunosorbent assay was developed to
measure specific antibody response in bovine sera to bovine
adenovirus type 3 (BA3), an etiologic agent of respiratory
disease causing economic losses annualy to the cattle
industry. Observed endpoint titers were determined using
the intersection point from optical density values of
serially diluted...
Fifteen adenoviruses from cattle, sheep, horses, and llamas were studied by virus neutralization to determine their degree of antigenic similarity. Prototype viruses included bovine adenoviruses species 1-8, ovine adenoviruses species 5 and 6, and equine adenovirus species 1. Unclassified viruses that were compared to the prototype viruses were isolated from...
A study was undertaken to determine the prevalence of virus infection in lambs undergoing respiratory tract disease (RTD). The investigation was divided into three study groups and consisted of lambs which were 4 to 5 months of age. In the first group (N=200), paired serum samples were taken from lambs...
Six 3 to 4 weeks old, cesarian-derived lambs were inoculated with ovine an adenovirus isolate 475N. Inoculated lambs showed moderate clinical signs of respiratory distress, conjunctivitis, and loose feces during the 10-day observation period. Virus was detected from nasal and conjunctival swabs starting on postinoculation day (PID) 2.
Virus was...