Doxorubicin is a widely used cancer therapeutic, but its effectiveness is limited by cardiotoxic side effects. Evidence suggests cardiotoxicity is due not to doxorubicin, but rather its metabolite, doxorubicinol. Identification of the enzymes responsible for doxorubicinol formation is important in developing strategies to prevent cardiotoxicity. In this study, the contributions...
The effects of several hormones and inducers of intracellular
messengers on plasminogen activator (PA) production and changes in
embryonic size by cultured bovine embryos were evaluated. Day 8 embryos
were cultured in Ham's F-12 with 1.5 mg/ml bovine serum albumin (BSA)
containing different levels of progesterone (P), estradiol -17fl (E₂),...
This study Is a rhetorical analysis of ten documents of Joseph
Smith, Jr's. early revelations spanning the period of 1829 to 1831
to determine the extent to which he used demagogic rhetoric in pursuit
of his goals. A definition of demagoguery is provided and is
applied in an analysis of...
Sodium dodecyl sulfate polyacrylamide gel electrophoresis and
zymography were used in two separate experiments to determine the tissue
source and type of plasminogen activator (PA) produced by bovine
blastocysts. Twelve-14 d blastocysts were collected at slaughter from
estrous synchronized, superovulated and artificially inseminated Holstein
cows. In Experiment I, blastocysts were...
The relationship between plasminogen activator (PA) production and cell stage, cell number and changes in overall diameter and zona pellucida thickness for bovine embryos developing in vitro was determined. Late morulae to blastocysts (n=80) were collected nonsurgically from naturally mated, estrous-synchronized and superovulated crossbred beef cows. Embryos were cultured, one...
The relationship between dose of follicle stimulating hormone (FSH) used to superovulate cattle embryo donors and embryo viability as measured by in vitro development and plasminogen activator (PA) production was determined. Late morulae to blastocysts were collected non-surgically from 12 superovulated crossbred beef cows. Embryos were cultured for 8 d...
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Alfred R. Menino, Jr.
The relationship between dose of follicle stimulating hormone (FSH) used
During early embryonic development, endodermal cells leave the inner cell mass (ICM) and migrate over an extracellular matrix (ECM), located on the blastocoelic side of the trophectoderm, to form a continuous layer of extraembryonic endoderm. Cell migration events depend on a family of cell surface proteins known as integrins that...
The type of plasminogen activator (PA) secreted by bovine embryos was identified. Day 12 to 14 blastocysts were collected at slaughter from estrous-synchronized, superovulated and naturally mated crossbred beef cows. Embryos were either left as intact embryos (E) or microdissected into component embryonic discs (ED) and trophoblastic vesicles (TV). Intact...
Two research projects comprise this thesis. The first project investigated modifying the hormone dosing strategy traditionally used in superovulating donor cows for an embryo collection to decrease drug costs without decreasing the number of high quality, transferable embryos recovered. The objective of this project was to evaluate the number and...