Batch cultures of Candidatus Pelagibacter ubique were grown under iron-, organosulfur-, and nitrogen-limiting conditions to understand how this ubiquitous marine bacterium responds to and interacts with environments where growth is limited by the availability of these nutrients. Global gene expression was monitored using microarrays and quantitative mass spectrometry to observe...
The first committed step in DNA biosynthesis occurs by direct reduction of
ribonucleotides. This reduction is catalyzed by ribonucleotide reductase (RNR), an
enzyme which uses a unique radical mechanism to facilitate the transformation. All four
DNA precursors are synthesized by a single enzyme. Therefore, an intricate pattern of
regulation has...
Iron is absolutely necessary for the maintenance, the defense, the differentiation and
last, but by no means least, the growth and cellular division of almost all living
organisms including Nitrosomonas europaea, an ammonia oxidizing bacterium. Given
the necessity of iron, it is not surprising that N. europaea evolved several irondependent...
Long-term stimuli of many systems leads to decreased cellular responsiveness, or desensitization. We characterized the desensitization of angiotensin II (Ang 11)-mediated inositol phospholipid (IP) hydrolysis in cultured human aortic smooth muscle cells (HASMC). Although it has been suggested that the desensitization induced by long-term Mg II exposure may result partially...
Lactococcus lactis subsp. cremoris Ropy 352 and L. lactis
subsp. cremoris Hollandicus produce an exopolysaccharide (EPS) that
imparts commercially desirable textural and rheological properties
to fermented milk products. This ropy phenotype is expressed under
specific environmental conditions. A mucoid EPS phenotype, also
expressed under specific environmental conditions, but not involved...
The transformation of 1,1,1 -trichioroethane (1,1,1 -TCA) and 1,1 -dichioroethene (1,1 -
DCE) was evaluated in a continuous flow column reactor using a mixed culture that grew
on butane. The column was packed with aquifer materials and groundwater obtained
from the in-situ bioremediation test site at Moffett Field, CA. The...
.............................................................33
Real Time PCR microbial analysis...................................................36
Summary of
The 1,1-dichloroethene (1,1-DCE) cometabolic transformation abilities of indigenous and bioaugmented microorganisms were compared in microcosms constructed with groundwater and aquifer solids from the Moffett Field site, CA. Microbial community structure in the microcosms and possible community shifts due to 1,1-DCE transformation stress was evaluated by terminal restriction fragment length polymorphism...
Fungi are capable of growth on a wide variety of carbon sources, both living and dead. They can produce an arsenal of enzymes and transporters for harvesting sugars, polysaccharides, amino acids, lipids and micronutrients from their environments [1]. Within the nucleus of a cell, transcription factors (TF) control whether genes...