Genetic studies of Tilletia spp. have been hampered by the lack
of genetic markers. The work discussed here is a first report of the
isolation of genetically marked strains of J. caries and their use in
wheat plant infection studies. Procedures for the isolation of auxotrophic and drug-resistant mutants in...
Five strains of the bean pathogen Pseudomonas syringae pv.
phaseolicola were analyzed for plasmid number, size, and relatedness.
Each strain contained one or two indigenous plasmids which ranged in
size from 6.8 to 151 kbp. BamHI and EcoRI restriction patterns revealed that total plasmid DNA from each strain had a...
A strain of the phytopathogenic bacterium Pseudomonas syringae
pv. phaseolicola, a pathogen of common bean Phaseolus vulgaris,
harbors a 150 kilobase pair (kb) plasmid pMC7105. In one strain,
LR719, pMC7105 is stably integrated in the bacterial chromosome.
Imprecise excision of the pMC7105 replicon has been shown to result
in the...
The objective of this study was to provide basic
information on the interaction of the bunt fungi
Tilletia caries and T. controversa with their host,
Triticum aestivum. Studies on the ability of haploid
strains to colonize wheat heads provided the basis for
experiments in which haploid strains were crossed in...
Electrophoretic karyotypes were obtained from intact cells of four wild
type strains of Tilletia caries and T. controversa, and 5 inter specific hybrid
progeny using clamped homogeneous electric field, pulsed field gel
electrophoresis (CHEF PFGE). Each karyotype was polymorphic and unique
relative to the other strains. However, the relative size...
Pulsed-field gel electrophoresis (PFGE) of the strains representing the 14 races of
U. hordei, the causal agent of covered smut on barley, revealed extensive polymorphism
in chromosome length and number. The purpose of this study was to determine by two
approaches the exact chromosome number for each U. hordei race,...
Sporulation in the yeast Saccharomyces cerevisiae involves
the process of meiosis which is accompanied by ascospore formation.
Using intact cells or spores, preliminary biochemical evidence is
given to identify ascospore wall components not found in vegetative
cell walls. Cell surface components are solubilized by treatment
with 8 M urea and...
Six plant pathogenic isolates of Pseudomonas
syringae pv. glycinea, the causal agent of bacterial
blight of Glycine max (L.) Merrill, and one isolate of
P. syringae pv. phaseolicola, the causal agent of halo
blight of Phaseolus vulgaris L., have been examined for
the presence of circular duplex deoxyribonucleic acid
(DNA)....