The cometabolic ability of Burkholderia vietnamiensis G4 grown on aromatic substrates was first assessed by resting suspended cells, and subsequently for cells encapsulated with and without slow release compounds (SRCs). In Chapter 3, benzyl alcohol was assessed as a food-grade growth substrate for promoting cometabolism of trichloroethene (TCE), via the...
Chlorinated aliphatic hydrocarbons (CAHs) like trichloroethene (TCE) were mostly used to replace drying cleaning fluids which contained hydrocarbons like benzene, and were highly flammable. Through improper disposal, storage, and spills, TCE and its downstream products, like cis-dichloroethene (cDCE) and vinyl chloride (VC), leached into groundwater and threaten human and environmental...
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MohammadAzizian, Committee Member, representing School of Chemical, Biological, and
As access to potable water grows scarce, there is an increasing reliance on alternative drinking water sources, namely groundwater (WHO, 2018). Chlorinated solvents, such as 1,1,1-trichloroethane (TCA) and 1,2-cis-Dichloroethene (cisDCE) are some of the most ubiquitous groundwater contaminants spread throughout the US. 1,4-dioxane (1,4D) was used primarily in the late...
CisCis-Dichloroethylene (ciscis-DCE), 1,1,1-Trichloroethane (1,1,1-TCA), and 1,4-Dioxane (1,4-D) are chlorinated solvents used as solvents and degreasers in industrial cleaning agents that have become common groundwater contaminants. To develop an effective treatment method for these compounds, polyvinyl alcohol (PVA) and sodium alginate (SA) hydrogel beads were co-entrappcapsulated with Rhodococcus rhodochrous Strain ATCC...
1,4-Dioxane (dioxane) and cis-dichloroethylene (cDCE) are compounds commonly found in industrial cleaning and degreasing agents that are frequently present as groundwater contaminants. In an effort to develop a more effective treatment method for these compounds, hydrogel beads were fabricated with either gellan gum or a combination of polyvinyl alcohol (PVA)...
Mixtures of 1,4-dioxane (1,4-D) and chlorinated aliphatic hydrocarbons (CAHs) are common and hazardous groundwater contaminants that can be challenging to address with traditional remediation strategies. Bioremediation through aerobic cometabolism has the potential to be an effective in-situ treatment strategy for these contaminants. Rhodococcus rhodochrous strain ATCC 21198 is capable of...
1,4-dioxane and chlorinated aliphatic hydrocarbons (CAHs) such as trichloroethylene (TCE) and 1,1-dichloroethene (1,1-DCE) are hazardous compounds commonly found in soil and groundwater. Bioremediation through aerobic cometabolism is a potential option for the remediation of these contaminated sites. The purpose of this study was to examine the use of different primary...
Chlorinated ethenes are common groundwater contaminants that may be treated through in-situ bioremediation. Relationships between the reducing environment, available electron donors and acceptors, reaction kinetics, and microbial community composition must be further understood to successfully engineer remediation schemes in the complex subsurface environment. This thesis work investigated the effect of...
Chlorinated solvents are common groundwater pollutants that often exist as mixtures resulting from the use of multiple solvents and their transformation into chlorinated daughter products. These sites are often co-contaminated with the solvent stabilizer 1,4-dioxane (1,4-D), which is not treated by traditional chlorinated solvent remediation techniques. Both 1,4-D and chlorinated...
Methods were developed for the co-encapsulation of slow release compounds (SRC) with viable microbial cells in alginate and gellan gum hydrogel beads, for the in-situ aerobic cometabolic treatment of groundwater contaminated with mixtures of 1,4-dioxane and chlorinated aliphatic hydrocarbons, which will be referred to as contaminants of concern (CoC). The...