Inflammation is one of the first, most important responses of the immune system when presented with infection and is mediated primarily by eicosanoids and cytokines. The goal of this research was to generate monoclonal antibodies against murine IL17C, a poorly characterized cytokine involved in inflammation and linked to inflammatory conditions....
Monoclonal antibodies (Mabs) were produced against the
myxosporean parasite, Ceratomyxa shasta, and were characterized
by indirect immunofluorescent techniques, western blot analysis,
and immunoelectron microscopy. The resulting Mabs reacted
specifically with the trophozoite and sporoblast stages of the
parasite, but not with the mature spore, indicating that certain
antigens are stage-specific....
Monoclonal antibodies were developed against USDA
challenge strain of infectious laryngotracheitis virus (ILTV).
Indirect immunofluorescence test was used to detect antibodies
in supernatants of hybridomas. Hybridoma cells were developed
by fusing Sp 2/0 myeloma cells with spleen cells obtained from
mice immunized four times with partially purified USDA
challenge strain...
Monoclonal antibodies were developed against serotype 3, P-1059 strain of Pasteurella multocida. Enzyme-linked immunosorbent assays (ELISA) were used to detect antibodies in supernatants of hybridoma cells to either a surface protective (2.5 S) antigen prepared from a 2.5% saline extract or lipopolysaccharide (LPS) prepared by the phenol-water method. Forty two...
Monoclonal antibodies (MCA) produced against infectious
laryngotracheitis virus (ILTV) of chickens reacted in
western blotting experiments with several different ILTV
protein bands in the absence of tunicamycin which inhibits
carbohydrate synthesis. Most of the MCA lost their
reactivity in western blotting experiments when extracts of
tunicamycin-treated ILTV CELC were used,...