Interactions between sediment bacteria and available phosphate
necessary for algal production in a eutrophic lake were studied.
Twenty percent of the organisms isolated solubilized phosphate forms
such as CaHPO₄, Ca₃(PO₄)₂, FePO₄, Mg₃(PO₄)₂ and Al₂(PO₄)₂.
Solubilization of phosphates occurred under conditions of aerobic
growth.
Upon quantitative analysis, some isolates were capable...
The vitamin B₁₂ concentrations in both the water and sediments
of Klamath Lake were found to vary significantly during a
one-year period. The patterns of B₁₂ distribution were remarkably
similar among the three main sites. In all cases there was an extremely
sharp gradation in concentration in relation to depth...
Washed cells of Vibrio marinus MP-1 grown at 15 C (organism's
optimum growth temperature) were employed in this study. These
cells were exposed to heat at and above their maximal growth temperature
(20 C) for one hour and tested manometrically at 15 C for their
ability to take up oxygen....
The addition of catalase to culture medium increased the period
of viability of Beggiatoa from one week to two months. Addition of
catalase also produced a marked increase in cell yield and enzyme
activity. The addition of cysteine or hydrogen sulfide to the growth
medium or the use of semi-solid...
Vibrio marinus MP-1 (ATCC 15381) deaminated nine of seventeen
amino acids tested with L-glutamine being deaminated to the greatest
and L-serine to the second greatest extent in one hour. The optimum
pH for the L-serine deamination was 8.4.
The response of washed cells to temperature on the deamination
of L-serine...
Vibrio marinus, strain MP-1, was shown to grow from pH 6.7
to 8.5, with an optimum at 7.3. Growth was inhibited below salinity
10%₀ and above 60%₀ . The upper temperature limit of
growth was 20 C, and growth was very rapid with aeration at 15 C.
Heating of the...
Four-fold partially purified glucose-6-phosphate dehydrogenase
(G-6-PDH) from the obligately psychrophilic marine bacterium
Vibrio marinus was effected by thermally induced leakage of the
enzyme, ammonium sulfate fractionation and column chromatography
on Sephadex G-200 gel.
The effects of temperature, pressure, salinity, and pH were
determined for the various degrees of enzyme purity....
Some enzymes of the citric acid cycle and glycolytic pathway
in cell-free extracts of Vibrio marinus MP-1 were compared for
thermal lability. After one hour of moderate temperature exposure,
enzymes of both pathways rapidly lost catalytic activity. For all
but one enzyme, 50 percent remaining activity occurred near an
averaged...
The marine psychrophilic bacterium Vibrio marinus MP-4
possessed a maximum temperature and hydrostatic pressure of
20 C and 425 atm for growth. The effects of temperatures of 21 and
25 C and hydrostatic pressures of 200, 400, 500, and 1,000 atm on
protein, RNA and DNA synthesis by V. marinus...