Russet Burbank, Russet Norkotah, and Shepody tubers infected with Potato virus Y strains (PVYO, PVYN:O, PVYNTN) were tested from storage at 4A degrees C at the initiation of sprouting and then the same tubers were tested again from storage at 4A degrees C seventy eight days later. Samples were taken...
A simple molecular tool was developed and tested to identify seven mealybug species found in North American vineyards: Pseudococcus maritimus Ehrhorn, Pseudococcus viburni (Signoret), Pseudococcus longispinus (Targioni-Tozzeti), Pseudococcus calceolariae (Maskell), Planococcus fleas (Signoret), Planococcus citri (Risso), and Ferrisia gilli Gullan. The developed multiplex PCR is based on the mitochondrial cytochrome...
• Premise of the study: The dramatic advances offered by modern DNA sequencers continue to redefine the limits of what can be accomplished in comparative plant biology. Even with recent achievements, however, plant genomes present obstacles that can make it difficult to execute large-scale population and phylogenetic studies on next-generation...
Full Text:
oligonucleotides or PCR
products spanning the target region); (2) has made the choice of whether to use
• Premise of the study: The dramatic advances offered by modern DNA sequencers continue to redefine the limits of what can be accomplished in comparative plant biology. Even with recent achievements, however, plant genomes present obstacles that can make it difficult to execute large-scale population and phylogenetic studies on next-generation...
• Premise of the study: The dramatic advances offered by modern DNA sequencers continue to redefine the limits of what can be accomplished in comparative plant biology. Even with recent achievements, however, plant genomes present obstacles that can make it difficult to execute large-scale population and phylogenetic studies on next-generation...
Full Text:
.
PCR‐BASED ENRICHMENT, SHORT TARGETS (0.5 kb targets), DIRECT
SEQUENCING
Assumptions: (1
• Premise of the study: The dramatic advances offered by modern DNA sequencers continue to redefine the limits of what can be accomplished in comparative plant biology. Even with recent achievements, however, plant genomes present obstacles that can make it difficult to execute large-scale population and phylogenetic studies on next-generation...
Full Text:
nuclear targets. These methods fall under four categories:
PCR-based enrichment, hybridization-based
• Premise of the study: The dramatic advances offered by modern DNA sequencers continue to redefine the limits of what can be accomplished in comparative plant biology. Even with recent achievements, however, plant genomes present obstacles that can make it difficult to execute large-scale population and phylogenetic studies on next-generation...
Phytophthora ramorum continues to cause extensive mortality of tanoaks in southwestern Oregon. Rain readily washes inoculum down through the canopy, causing new infections on the lower parts of the tree and neighboring host plants. Although this aspect of dispersal is well understood, the relative importance of infested soil and leaf...
Since around 1923 Port-Orford-cedar (Chamaecyparis lawsoniana (A. Murray)
Parl.) has been affected by Phytophthora root disease caused by the virulent introduced
pathogen Phytophthora lateralis. A systematic resistance testing and breeding program
located at the USDA Forest Service Dorena Genetic Resource Center in Cottage Grove,
Oregon was initiated in 1997 with...
Since around 1923 Port-Orford-cedar (Chamaecyparis lawsoniana (A. Murray)
Parl.) has been affected by Phytophthora root disease caused by the virulent introduced
pathogen Phytophthora lateralis. A systematic resistance testing and breeding program
located at the USDA Forest Service Dorena Genetic Resource Center in Cottage Grove,
Oregon was initiated in 1997 with...