The halophilic alkaline phosphatase of Halobacterium
salinarium has been purified to a specific activity of 3,000-3,200
units per mg of protein. The purification scheme consisted of
acetone and ammonium sulfate fractionations, and molecular sieving
and adsorption chromatography on Sephadex G -50. The enzyme was
stabilized during purification by the presence...
Enzymes play an important role in the environment, they breakdown natural-occurring and anthropogenic molecules so that they can be transported into cells and utilized. Enzyme assays are routinely used in soil science and oceanography to measure the activities of specific processes and to serve as general indicators of microbial activity....
Published October 1958. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Alkaline phosphatase (ALP) is an enzyme present in intestinal mucosa, bile, bone and renal tubule cells. Bile acids have been shown to decrease ALP activity from bone and kidney but not those from intestinal origin. This action can be mimicked in serum and peritoneal fluid samples by the use of...
Aerobic organisms have evolved many sensory mechanisms that allow
response to oxidants in the environment. One area of interest is the relationship
between the activity of the tumor suppressor protein p53 and the redox state of
thioredoxin. Human p53 activity is severely compromised in budding yeast lacking
thioredoxin reductase. Evidence...