Under defined cultural conditions, large populations of zygotes
of the brown algae Fucus vesiculosus L. and F. distichus L. Powell
synchronously form a cell wall shortly after fertilization. Intact cell
walls, free of cytoplasm and maintaining shape properties of early
stages of embryo development, have been isolated from these zygotes....
Fucus distichus L. Powell zygotes were used to investigate
cell wall synthesis and development and to construct a model of
cell wall structure.
Alginate synthesis was investigated. Zygotes were cultured
in seawater containing ¹⁴C-bicarbonate. Alginate represented
12%, 37%, and 1.7% of the label recovered from whole zygotes,
wall, and cytoplasm,...
Hop cell wall material (CWM) extracted from hop leaves (hamulus lupulus) was
purified and characterized. The total sugar content, uronic acid content and
monosaccharide composition of the CWM were determined. Galacturonic acid is the
major component in the CWM. A mixture of unsaturated oligogalacturonides were
released from purified hop CWM...
This work is an attempt to develop a simple physical description for the initial
interactions between a bacterial cell and a substrate surface. This is done by
adopting a view that regards bacterial surface biomolecules not as secondary and
add-on contributors to the potential energy function that characterizes the
Excised apical stem tips (2-5 mm) of grape cultivars grew well
on Murashige and Skoog's (1962) medium for tobacco callus. Additions
of glycine were not essential but gave a slight stimulus to growth.
Naphthalene acetic acid slightly reduced growth rates but reductions
were not statistically significant. Kinetin and coconut milk...
The overall focus of this thesis is on the distribution of specific lipids and membrane proteins of the external and internal membranes of plant cells, in the context of the roles that those lipids and proteins may play in microbe-plant interactions. The work includes the development of several new tools,...