Vegetative propagation allows the amplification of selected genotypes for research,
breeding, and commercial planting. However, efficient in vitro regeneration and
genetic transformation remains a major obstacle to research and commercial
application in many plant species. Our aims are to improve knowledge of gene
regulatory circuits important to meristem organization, and...
Society uses massive quantities of wood fiber in production of paper, and demand for fiber is projected to increase further as production of biofuels from fermentation of plant cellulosic materials increases. Because these end uses generally require the costly step of removing of lignin, wood with reduced or more easily...
The Polycomb group (PcG) of genes of Drosophila melanogaster Meigen are involved in developmental regulation through chromatin remodeling. Homologs of PcG genes have been identified in both plant and animal species. In this study, we detail the identification and characterization of Triticum enhancer of zeste 1 (Tez1), a homolog of...
The transcriptional promoter sequences for the Rhizobium meliloti
nitrogen fixation genes nifA and nifB were cloned to a β-galactosidase gene
fusion plasmid vector and transferred by homologous recombination to a
specialized transducing phage. The promoter fusions were then transduced
to a recombination deficient strain of Escherichia coli as single-copy
lysogens...
Lactococcus lactis subsp. cremoris Ropy 352 and L. lactis
subsp. cremoris Hollandicus produce an exopolysaccharide (EPS) that
imparts commercially desirable textural and rheological properties
to fermented milk products. This ropy phenotype is expressed under
specific environmental conditions. A mucoid EPS phenotype, also
expressed under specific environmental conditions, but not involved...
In order to maintain genetic stability in eukaryotes, tight regulation of the relative sizes of deoxyribonucleoside triphosphate (dNTP) levels inside the cell is essential for optimal fidelity of DNA replication. Ribonucleotide reductase (RNR) is the enzyme responsible for proportional production of DNA precursors. Studies on regulation of this enzyme, the...
Pseudomonas aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals such as those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell communication mechanism known as quorum sensing (QS) to coordinate virulence gene expression and biofilm formation. It has three interconnected QS systems, namely...
Fungi are capable of growth on a wide variety of carbon sources, both living and dead. They can produce an arsenal of enzymes and transporters for harvesting sugars, polysaccharides, amino acids, lipids and micronutrients from their environments [1]. Within the nucleus of a cell, transcription factors (TF) control whether genes...
Skin is the largest organ in the body that protects the organism from environmental, chemical and physical traumas of each passing day. The protective skin epidermal permeability barrier (EPB) is formed within the exterior layers of the epidermis, which are regularly sloughed off and repopulated by movement of inner cells....