Aims:
To design and validate a colorimetric loop-mediated isothermal amplification assay for rapid detection of Phytophthora infestans DNA.
Methods and Results:
Two sets of loop-mediated isothermal amplification (LAMP) primers were designed and evaluated for their sensitivity and specificity for P. infestans. ITSII primers targeted a portion of the internal transcribed...
In eukaryotes, RNA silencing pathways utilize 20-30-nucleotide small RNAs to regulate gene expression, specify and maintain chromatin structure, and repress viruses and mobile genetic elements. RNA silencing was likely present in the common ancestor of modern eukaryotes, but most research has focused on plant and animal RNA silencing systems. Phytophthora...
Modulation of gene expression through RNA interference is well conserved in eukaryotes and is involved in many cellular processes. In the oomycete Phytophthora, research on the small RNA machinery and function has started to reveal potential roles in the pathogen, but much is still unknown. We examined Argonaute (AGO) homologs...
Gene regulation by small RNA pathways is ubiquitous among eukaryotes, but little is known about small RNA pathways in the Stramenopile kingdom. Phytophthora a genus of filamentous oomycetes, contains many devastating plant pathogens, causing multibillion-dollar damage to crops, ornamental plants, and natural environments. The genomes of several oomycetes including Phytophthora...
Root rot of raspberry (Rubus idaeus), thought to be primarily caused
by Phytophthora rubi, is an economically important disease in the
western United States. The objectives of this study were to determine
which Phytophthora species are involved in root rot, examine the efficacy
of different isolation methods (cane, root, and...
Radio astronomy allows observations of unique objects and phenomena relating to the electric and magnetic fields of celestial objects. Radio astronomy can be preformed at any time of day and is less beholden to atmospheric conditions than optical astronomy. A radio telescope designed to receive radiation at 1.421 GHz, the...
Microcomputers are being used in teaching and learning
mathematics. This paper examines ways that computers can be used
to enhance teaching in precalculus mathematics. The advantages of
computers discussed are: 1) instant access to graphs, 2) examples
that are not oversimplified to make computations manageable, 3)
encouragement of hypothesizing and...
In his works, A Portrait of the Artist as a Young Man (1916) and Ulysses
(1922), James Joyce demonstrates what he perceives to be the paralyzing effects of
those institutionalized religions that sit at the center of cultures. Drawing on Michel
Foucault's analysis of institutional dressage as well as his...
Phytophthora infestans, the cause of the devastating late blight disease of potato and tomato, exhibits a clonal reproductive lifestyle in North America. Phenotypes such as fungicide sensitivity and host preference are conserved among individuals within clonal lineages, while substantial phenotypic differences can exist between lineages. Whole P. infestans genomes were...