There is good evidence that the infection with avian myeloblastosis
virus BAI strain A causes myeloblasts to retain their primitive
nature. These cells are able to multiply and produce virus at a relatively
constant rate. From these observations, a major problem develops
in the study of the virus-induced leukemia, that...
miRNA-guided cleavage initiates entry of primary transcripts into the trans-acting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA POLYMERASE6 (RDR6), DICER-LIKE 4 (DCL4), and SUPPRESSOR OF GENE SILENCING3 (SGS3). Arabidopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173- guided initiation cleavage on the 5’ side of the siRNA-generating...
RNAs play important roles in the central dogma of molecular biology, and are involved in multiple biology processes such as chromatin modification, transcriptional interference and translation initiation. The functions of RNAs, especially non-coding RNAs, are highly related to its secondary structures, therefore computational methods for RNA structure prediction are of...
Messenger RNA (mRNA) therapy has massive potential to treat various genetic disorders by providing effective proteins to the diseased cells and tissues. Its therapeutic applications include protein replacement, genome editing, and immunotherapy. For successful mRNA treatment, mRNA must be delivered to the cytosol of target cells. One of the leading...
RNA isolated from BAI strain A (Myeloblastosis) Avian Tumor
Virus can be fractionated into two fractions on a sucrose density
gradient, one of higher molecular weight and one of low molecular
weight. The low molecular weight (LMW) virus RNA was found to
chromatograph the same as E. coli tRNA on...
Base-pairing interaction are the fundamental blocks of an RNA structure. With increased ability to determine the structure of RNA molecules, an accurate and informative way of classifying these interactions would help to study RNA structure at many levels. Apart from the well-known complementary base pairs and wobble pairs, other interactions...
RNA-Sequencing (RNA-Seq) has rapidly become the de facto technique in transcriptome studies. However, established statistical methods for analyzing experimental and observational microarray studies need to be revised or completely re-invented to accommodate RNA-Seq data's unique characteristics. In this dissertation, we focus on statistical analyses performed at two particular stages in...
ABSTRACT
Eukaryotes produce functionally diverse classes of
small RNAs (20–25 nt). These include microRNAs
(miRNAs), which act as regulatory factors during
growth and development, and short-interfering
RNAs (siRNAs), which function in several epigenetic
and post-transcriptional silencing systems. The
Arabidopsis Small RNA Project (ASRP) seeks to characterize
and functionally analyze the...
Background: Short RNAs, and in particular microRNAs, are important regulators of gene
expression both within defined regulatory pathways and at the epigenetic scale. We investigated
the short RNA (sRNA) population (18-24 nt) of the transcriptome of green leaves from the
sequenced Populus trichocarpa using a concatenation strategy in combination with...
Avian myeloblastosis virus RNA was fractionated into a high
molecular weight RNA and a low molecular weight RNA fraction by
sucrose density centrifugation. The larger RNA component had a
sedimentation constant of 65S and the low molecular weight RNA
about 4S. The low molecular RNA has two biological properties of...