Index Catalog // ScholarsArchive@OSU

Molecular cloning of infectious pancreatic necrosis virus and characterization of the coding capacity of the complementary DNA

Creator:: Huang, Manley T. F.
Abstract:: The two segments of double-stranded RNA (dsRNA) from infectious pancreatic necrosis virus (IPNV) were cloned into plasmid vector pUC8. Two distinct sets of overlapping clones were identified by restriction enzyme and Southern blot analysis. Each of these sets was shown, by Northern blot analysis, to be exclusively related to either...
Resource Type:: Dissertation
Full Text:: The two segments of double-stranded RNA (dsRNA) from infectious pancreatic necrosis virus (IPNV) were

Investigation of cis-acting RNA element role in bovine viral diarrhea virus replication

Creator:: Ly, David
Abstract:: Bovine viral diarrhea virus (BVDV) is an enveloped, single-stranded positive-sense RNA virus. The BVDV genome is about 12.5 kb long and consists of a single open reading frame (ORF) flanked by untranslated regions at the 5' and 3' ends (5'-UTR and 3'- UTR, respectively). To investigate the role of cis-sequences...
Resource Type:: Masters Thesis
Full Text:: . Title: Investigation of Cis-acting RNA Element Role in Bovine Viral Diarrhea Virus Replication

Genetic requirements for the assembly and cell-to-cell movement of the beet yellows virus

Creator:: Alzhanova, Dina
Abstract:: Beet yellows virus (BYV) is a filamentous, positive-strand RNA virus that belongs to the family Closteroviridae. BYV particles encapsidate a 15.5 kb RNA and posses complex polar architecture. A long virion body is formed by the major capsid protein(CP), whereas the minor capsid protein (CPm) assembles a short tail that...
Resource Type:: Dissertation
Full Text:: yellows virus (BYV) is a filamentous, positive-strand RNA virus that belongs to the family

The roles of turnip yellow mosaic virus genes in virus replication

Creator:: Weiland, John J.
Abstract:: Turnip yellow mosaic virus is a monopartite, plus sense RNA virus infecting the Cruciferae, and is a model system for the study of RNA virus replication. A cDNA clone (pTYMC) representing an infectious RNA genome of the European isolate of TYMV was constructed and used to assess the importance of...
Resource Type:: Dissertation
Full Text:: . Abstract approved: Theo W. Dreher Turnip yellow mosaic virus is a monopartite, plus sense RNA virus

The biological significance of low molecular weight RNA associated with tumor virus

Creator:: Carnegie, John William
Abstract:: RNA isolated from BAI strain A (Myeloblastosis) Avian Tumor Virus can be fractionated into two fractions on a sucrose density gradient, one of higher molecular weight and one of low molecular weight. The low molecular weight (LMW) virus RNA was found to chromatograph the same as E. coli tRNA on...
Resource Type:: Masters Thesis
Full Text:: The Biological Significance of Low Molecular Weight RNA

Studies on the transforming gene of avian myeloblastosis virus

Creator:: To, Richard Yue-leung
Resource Type:: Dissertation
Full Text:: complementary to genomic RNA from avian myeloblastosis (AMV) virus was fractionated by exhaustive hybridization

Molecular characterization of infectious hematopoietic necrosis virus transcription and genome organization

Creator:: Kurath, Gael
Abstract:: The transcription process of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus of salmonid fish, was studied both in vivo and in vitro. Polyadenylated RNA from IHNV infected-salmon cells was resolved electrophoretically into five bands, one of which was found to contain two mRNA species. The molecular weights and intracellular molar...
Resource Type:: Dissertation
Full Text:: . Polyadenylated RNA from IHNV infected-salmon cells was resolved electrophoretically into five bands, one of

Molecular characterization of the proteinase and RNA-dependent RNA polymerase of Infectious Pancreatic Necrosis Virus, a fish birnavirus

Creator:: Mason, Carla L.
Abstract:: The A segment of infectious pancreatic necrosis virus (IPNV) is expressed as a polyprotein encoding three primary gene products, VP2, NS and VP3, from a large open reading frame. The nucleotide sequence for the A segment of the Sp isolate of IPNV was determined. The NS protein is the putative...
Resource Type:: Dissertation
Full Text:: Molecular characterization of the proteinase and RNA

Adenovirus complementary strand synthesis in vivo

Creator:: Wang, Kai, 1955-
Abstract:: Replication of plasmid molecules containing a single adenovirus terminal sequence and specially arranged inverted repeat sequences were studied. When the plasmids were linearized to expose the adenovirus terminal sequence and transfected into 293 cells with helper adenovirus DNA, a larger molecule was detected. The new molecule contained adenovirus terminal sequences...
Resource Type:: Dissertation
Full Text:: activity for proofreading capability (52). But unlike other DNA polymerases, it elongates RNA-primed DNA

Characterization of Baculovirus genes involved in genome replication and processing

Creator:: Vanarsdall, Adam L.
Abstract:: The Baculoviridae comprise a diverse group of occluded DNA viruses that contain large double-stranded DNA genomes of 80 - 180 kb and may encode up to 180 gene products. To understand how baculoviruses replicate and process their genomes and the gene products that are involved in these events, a series...
Resource Type:: Dissertation
Full Text:: replication assay……………....……………………...…………56 Total RNA purification/ Northern blotting…………………….……..57 Virus

ScholarsArchive@OSU

Molecular cloning of infectious pancreatic necrosis virus and characterization of the coding capacity of the complementary DNA

Investigation of cis-acting RNA element role in bovine viral diarrhea virus replication

Genetic requirements for the assembly and cell-to-cell movement of the beet yellows virus

The roles of turnip yellow mosaic virus genes in virus replication

The biological significance of low molecular weight RNA associated with tumor virus

Studies on the transforming gene of avian myeloblastosis virus

Molecular characterization of infectious hematopoietic necrosis virus transcription and genome organization

Molecular characterization of the proteinase and RNA-dependent RNA polymerase of Infectious Pancreatic Necrosis Virus, a fish birnavirus

Adenovirus complementary strand synthesis in vivo

Characterization of Baculovirus genes involved in genome replication and processing

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