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Vaccinia virus ribonucleotide reductase : regulation of the gene products and characterization of the recombinant small subunit protein

Creator:

Howell, Meredith L.

Abstract:

Ribonucleotide reductase is a remarkable enzyme that catalyzes the rate-limiting step in the synthesis of the 2'-deoxynucleoside triphosphates. The intent of this project was to characterize the ribonucleotide reductase encoded by the orthopoxvirus, vaccinia. The first objective was to study the structural and functional features of the viral small subunit...

Resource Type:

Dissertation

Full Text:

the gene products and characterization of the recombinant small subunit protein AN ABSTRACT OF THE
The adsorption of human recombinant factor VIII in the presence of the nonionic triblock surfactant Pluronic® F-68 at the air-water interface

Creator:

Alkhatib, Aveen K.

Abstract:

The adsorption behavior of a human recombinant Factor VIII (rFVIII) in the presence of the nonionic, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock surfactant Pluronic® F-68 was evaluated by dynamic air-water tensiometry. Surface tension kinetics were recorded for F-68 in the absence of protein at concentrations ranging from 0 to 10,000...

Resource Type:

Masters Thesis

Full Text:

CHAPTER 3 MATERIALS AND METHODS 3.1 Preparation of protein, surfactant and buffer The recombinant