Double-stranded cDNA was prepared from infectious hematopoietic
necrosis virus mRNA and cloned into the plasmid vector pUC8. A cDNA
clone (pG8) containing the entire coding region for the glycoprotein
(G-protein) of infectious hematopoietic necrosis virus was selected
by hybridization to a ³²P-labeled probe. The restriction map and
nucleotide sequence of...
Complementary DNA (cDNA) clones were generated from the RNA genome
of infectious hematopoietic necrosis virus (IHNV) by using random DNA
oligomers to prime first strand synthesis. These clones were mapped to
their respective locations on the genome and used to determine the
nucleotide sequence of the nucleocapsid (N) gene and...
The glycoprotein, G, of infectious hematopoietic necrosis virus, IHNV,
was shown to be the viral protein which elicits and reacts with neutralizing
antibodies. The G protein also confers protective immunity on immunized
susceptible salmonid hosts to IHNV. The other three structural proteins of
IHNV, N, Ml, and M2, do not...
The development of an effective infectious
hematopoietic necrosis virus (IHNV) vaccine depends on a
better understanding of the antigenic structure and
variation among isolates. Three objectives to elucidate
this were: (1) to generate a panel of antigenic
derivatives of the IHNV glycoprotein by monoclonal
antibody-mediated selection of neutralization-resistant
variant viruses,...
The complete genome of snakehead rhabdovirus (SHRV) was cloned and
molecularly characterized. This was initially accomplished through the sequence
determination of its glycoprotein gene and the phylogenetic analysis of this gene with
orthologous genes from other rhabdoviruses. The phylogenetic analysis revealed that
SHRV groups with viruses of the genus Novirhabdovirus....
A characterization of the antigenic determinants (epitopes) of the glycoprotein (G) of infectious hematopoietic necrosis virus (IHNV) was made with different regions of the G gene expressed in Escherichia coli. A cDNA copy of the G gene was divided into four fragments after Taq I digestion and these fragments were...
The virulence of selected isolants of infectious hematopoietic
necrosis virus (IHNV) were differentiated and correlated with
electrophoretic mobilities of virion structural proteins by sodium
dodecylsulfate polyacrylamide gel electrophoresis. A type 1
electropherotype of IHNV was more virulent for kokanee salmon
(Oncorhynchus nerka) and a type 2 strain more virulent for...