The solution structure determination of DNA molecules has been an
important part of structural biology. NMR solution structures are a
complement to structures solved via X-ray crystallography; the two methods
are the only ways of obtaining three dimensional coordinates of
macromolecules. Because of the nature of the molecule, the solution...
The cytolytic properties of the mosquitocidal 25 Kd protein deltaendotoxin of Bacillus thuringiensis subsp. israelensis (Bti) was studied using the human erythrocyte as a target cell. Both a fixedtime and a kinetic hemolytic bioassay were developed in order to screen a variety of treatments for their influence on the toxinerythrocyte...
Quinolinate (QUIN) is an endogenous neurotoxic product of the kynurenine
pathway of tryptophan metabolism. Quinolinate is a selective agonist of the NMDA
family of glutamate receptors and, as such, may play a role in the pathology of a number
of major central nervous system (CNS) disorders. These include both chronic...
Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a
complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases)
enzymes. One approach to separating these enzymes is affinity chromatography. A new
ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose.
The property of APTC in affinity chromatography is demonstrated using Trichoderma...
In this study, we have characterized a series of patient Abdominal Aortic
Aneurysms (AAAs) based on their geometric parameters, wall pressure
distributions and ages. Abdominal aortic aneurysms (AAAs) are localized aortic
dilations that have been estimated to occur in 5 to 7 percent of the U.S. population
over age 60....
This is the first study to explore the ability of an enzyme to recognize and repair
spontaneous age-dependent damage to its own sequence. Protein (D-aspartyl/L-isoaspartyl)
carboxyl methyltransferase (PCM) is known to repair damage that arises
from a spontaneous isomerization of aspartyl and asparaginyl residues in other proteins
during aging. As...