A comparative study was made on the effect of urea denaturation
of bovine serum albumin (BSA), polyglycyl BSA and poly-L-phenylalanyl
BSA. The denaturation process was followed by ultraviolet
spectrophotometry and optical rotation.
A blue shift in the absorption maximum at 278 mμ was observed
in urea denatured proteins, In 7M...
A heat stable derivative of bovine serum albumin containing
300 residues of glycine added to 30 sites on the surface of the protein
has been prepared. This derivative may be heated to 100° C for
prolonged periods of time without aggregation. By comparison,
native BSA aggregates at 62° C under...
The thermal denaturation of proteins has been extensively studied using several methods including differential scanning calorimetry (DSC). A custom-built optical system was used to study thermal effects on protein as an alternative method to DSC measurements. It was used to investigate the thermal stability of bovine serum albumin (BSA) with...