Index Catalog // ScholarsArchive@OSU

SiddensLisbethEnvMolecularToxicMammalianFlavin-Containing.pdf

Creator:: Siddens, Lisbeth K., Krueger, Sharon K., Henderson, Marilyn C., and Williams, David E.
Abstract:: Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and 3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂ upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production. Two common FMO2 (the major...
Full Text:: (FMO) as a Source of Hydrogen Peroxide Lisbeth K. Siddensa, Sharon K. Kruegerb, Marilyn C

SiddensLisbethEnvMolecularToxicMammalianFlavin-Containing_Figures1-9.zip

Creator:: Siddens, Lisbeth K., Krueger, Sharon K., Henderson, Marilyn C., and Williams, David E.
Abstract:: Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and 3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂ upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production. Two common FMO2 (the major...

Mammalian Flavin-Containing Monooxygenase (FMO) as a Source of Hydrogen Peroxide

Creator:: Siddens, Lisbeth K., Krueger, Sharon K., Henderson, Marilyn C., and Williams, David E.
Abstract:: Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and 3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂ upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production. Two common FMO2 (the major...
Resource Type:: Article

Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures

Creator:: Larkin, Andrew, Siddens, Lisbeth K., Krueger, Sharon K., Williams, David E., and Baird, William M.
Abstract:: Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin...
Resource Type:: Research Paper
Full Text:: with environmental PAH mixtures Andrew Larkina,b, Lisbeth K. Siddensb, Sharon K. Kruegerc, David E

MicroRNAs miR-221 and miR-181c regulation of liver tumor growth and metastasis in mouse progeny from Dibenzo[def,p]chrysene dosed mothers fed a sulforaphane diet

Creator:: Meedom, Chelsea, Siddens, Lisbeth K., Löhr, Christiane V., and Williams, David E.
Abstract:: Dibenzo[def,p]chrysene (DBC) is a polycyclic aromatic hydrocarbon (PAH) and an IARC classified 2A probable human carcinogen. Mouse progeny transplacentally exposed to DBC have been shown to develop lung and liver tumors, with liver tumors developing predominantly in males later in life. Sulforaphane (SFN) is an isothiocyanate that plays a crucial...
Resource Type:: Poster
Full Text:: Fed a Sulforaphane Diet Chelsea Meedom1, Lisbeth K. Siddens1, Christiane V. Löhr3, David E

Application of a fuzzy neural network model in predicting polycyclic aromatic hydrocarbon-mediated perturbations of the Cyp1b1 transcriptional regulatory network in mouse skin

Creator:: Larkin, Andrew, Siddens, Lisbeth K., Krueger, Sharon K., Tilton, Susan C., Waters, Katrina M., Williams, David E., and Baird, William M.
Abstract:: Polycyclic aromatic hydrocarbons (PAHs) are present in the environment as complex mixtures with components that have diverse carcinogenic potencies and mostly unknown interactive effects. Non-additive PAH interactions have been observed in regulation of cytochrome P450 (CYP) gene expression in the CYP1 family. To better understand and predict biological effects of...
Resource Type:: Article
Full Text:: Andrew Larkin a,b,c, Lisbeth K. Siddens a,c, Sharon K. Krueger c,d, Susan C. Tilton c,e, Katrina M

Mechanism-based classification of PAH mixtures to predict carcinogenic potential

Creator:: Tilton, Susan C., Siddens, Lisbeth K., Krueger, Sharon K., Larkin, Andrew J., Löhr, Christiane V., Williams, David E., Baird, William M., and Waters, Katrina M.
Abstract:: We have previously shown that relative potency factors and DNA adduct measurements are inadequate for predicting carcinogenicity of certain polycyclic aromatic hydrocarbons (PAHs) and PAH mixtures, particularly those that function through alternate pathways or exhibit greater promotional activity compared to benzo[a]pyrene (BaP). Therefore, we developed a pathway based approach for...
Resource Type:: Article
Full Text:: Susan C. Tilton* ,†,1 , Lisbeth K. Siddens* ,† , Sharon K. Krueger* ,‡ , Andrew J. Larkin* ,† , 3

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure4A).pdf

Creator:: Henderson, Marilyn C., Siddens, Lisbeth K., Krueger, Sharon K., Stevens, J. Fred, Kedzie, Karen, Fang, Ken, Heidelbaugh, Todd, Phong Nguyen, Chow, Ken, Garst, Michael, Gil, Daniel, and Williams, David E.
Abstract:: Mammalian flavin-containing monooxygenase (FMO) is active towards many drugs with a heteroatom having the properties of a soft nucleophile. Thiocarbamides and thiones are S-oxygenated to the sulfenic acid which can either react with glutathione and initiate a redox-cycle or be oxygenated a second time to the unstable sulfinic acid. In...

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure3C).pdf

Creator:: Henderson, Marilyn C., Siddens, Lisbeth K., Krueger, Sharon K., Stevens, J. Fred, Kedzie, Karen, Fang, Ken, Heidelbaugh, Todd, Phong Nguyen, Chow, Ken, Garst, Michael, Gil, Daniel, and Williams, David E.
Abstract:: Mammalian flavin-containing monooxygenase (FMO) is active towards many drugs with a heteroatom having the properties of a soft nucleophile. Thiocarbamides and thiones are S-oxygenated to the sulfenic acid which can either react with glutathione and initiate a redox-cycle or be oxygenated a second time to the unstable sulfinic acid. In...

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure3B).pdf

Creator:: Henderson, Marilyn C., Siddens, Lisbeth K., Krueger, Sharon K., Stevens, J. Fred, Kedzie, Karen, Fang, Ken, Heidelbaugh, Todd, Phong Nguyen, Chow, Ken, Garst, Michael, Gil, Daniel, and Williams, David E.
Abstract:: Mammalian flavin-containing monooxygenase (FMO) is active towards many drugs with a heteroatom having the properties of a soft nucleophile. Thiocarbamides and thiones are S-oxygenated to the sulfenic acid which can either react with glutathione and initiate a redox-cycle or be oxygenated a second time to the unstable sulfinic acid. In...

ScholarsArchive@OSU

SiddensLisbethEnvMolecularToxicMammalianFlavin-Containing.pdf

SiddensLisbethEnvMolecularToxicMammalianFlavin-Containing_Figures1-9.zip

Mammalian Flavin-Containing Monooxygenase (FMO) as a Source of Hydrogen Peroxide

Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures

MicroRNAs miR-221 and miR-181c regulation of liver tumor growth and metastasis in mouse progeny from Dibenzo[def,p]chrysene dosed mothers fed a sulforaphane diet

Application of a fuzzy neural network model in predicting polycyclic aromatic hydrocarbon-mediated perturbations of the Cyp1b1 transcriptional regulatory network in mouse skin

Mechanism-based classification of PAH mixtures to predict carcinogenic potential

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure4A).pdf

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure3C).pdf

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure3B).pdf

Limit your search

Academic Affiliation

Advisor

Commencement Year

Committee Member

Conference Name

Creator

Contributor

Date

Decade

Degree Field

Degree Level

Degree Name

Language

Non-Academic Affiliation

Peer Reviewed

Resource Type

Rights Statement

Search Constraints

Search Results

Limit your search