Gene flow is a major evolutionary force and an important factor in the breeding and conservation of forest trees. I studied the applicability of SSR markers for measuring pollen-mediated gene flow (i.e., pollen flow) in Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco). I developed SSR markers, tested alternative approaches for measuring pollen...
Comparative analysis of multiple angiosperm genomes has implicated gene duplication in the expansion and diversification of many gene families. However, empirical data and theory suggest that whole-genome and small-scale duplication events differ with respect to the types of genes preserved as duplicate pairs. We compared gene duplicates resulting from a...
The increased US domestic production of surimi from the US Pacific Coast after 1977 is a major consequence of the US's extended fisheries jurisdiction to 200 nautical miles. It is only one consequence, however, whose nature can best be understood by examining the surimi market in the context of what...
•Plant population genomics informs evolutionary biology, breeding, conservation and bioenergy feedstock development. For example, the detection of reliable phenotype–genotype associations and molecular signatures of selection requires a detailed knowledge about genome-wide patterns of allele frequency variation, linkage disequilibrium and recombination.
•We resequenced 16 genomes of the model tree Populus trichocarpa...
In the interest of meeting multiple forest management goals that include maintenance of wildlife, particularly cavity-nesting birds, uneven-aged silvicultural treatments are used increasingly in the Pacific Northwest. However, questions remain regarding the responses of cavity-nesting birds and residual green trees to different harvest intensities and patterns. To study these issues,...
Sphaerulina musiva is a fungal pathogen that causes Septoria leaf spot and stem canker on susceptible Populus species and hybrids. In this thesis, host and pathogen contributions to Septoria stem canker disease were explored through: 1) a greenhouse study associating Septoria stem canker phenotypes with molecular markers from a genetic...
In forest trees from temperate and boreal regions, cold acclimation is an important
adaptive trait that involves changes in gene expression and physiology. Genecological, quantitative genetic, and QTL studies have been used to study the genetics of cold acclimation in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), but the genes responsible for...
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A SNP resource for Douglas-fir: de novo transcriptome assembly and SNP detection and validation
Author(s): Howe, GT (Howe, Glenn T.)[ 1 ] ; Yu, JB (Yu, Jianbin)[ 1 ] ; Knaus, B (Knaus, Brian)[ 2 ] ; Cronn, R (Cronn, Richard)[ 2 ] ; Kolpak,...
Stable expression of transgenes is required for commercial uses of genetically engineered trees. To better understand the stability of transgene expression under field conditions, we studied transgene expression and RNA interference (RNAi)-induced transgene suppression in 2,480 transgenic poplars (460 transgene insertion events) over three years. Stability of expression was assessed...
This dissertation consists of four studies of Populus and Eucalyptus biotechnology and genomic science: 1) induction of floral sterility by tapetal expression of the ribonuclease Barnase in Populus; 2) CRISPR Cas9-mediated gene editing targeting LEAFY (LFY) and AGAMOUS (AG) homologs in Populus; 3) induction of floral sterility by CRISPR Cas9...