The need for better procedures for detecting Staphylococcus
aureus prompted an investigation to improve coagulase testing. The
rates of coagulase clotting were studied by using varying concentrations
of trypsin inhibitor and coagulase-reacting factor (CRF). Egg
white trypsin inhibitor activated coagulase clotting when added to a
final concentration between 2 and...
A selective, differential plating medium for the enumeration of
coagulase-positive staphylococci was developed. The ingredients of
this medium were analysed for their effect on normal, heat treated
and frozen staphylococcal cells. The nutrient base consisted of 10 gm
Tryptone, 5 gm Beef Extract, 1 gm Yeast Extract and 17 gm...
A method has been presented for the observation of a large
number of enterotoxin A producers. Production of a relatively concentrated
toxin is achieved by the sac culture method. Further concentration,
if needed, is carried out by PM-10 membrane ultrafiltration
after centrifugation. The work is further improved by
trypsinization of...
The effect of different oxygen atmospheres on growth, energy
charge and enterotoxin production of Staphylococcus aureus was
investigated. Three atmospheres, air, 5% oxygen in nitrogen and
20% oxygen in nitrogen, were tested on three strains producing
enterotoxins A, B and C. Colony forming units (CFU), dry weight,
dissolved oxygen, pH,...
Growth and enterotoxin production of Staphylococcus aureus in papain-treated beef and ham were studied. In addition to an untreated control, four treatments were used in the beef experiment including two levels of papain, a commercial tenderizer preparation, and commercially tenderized beef. Two levels of papain treatment and an untreated control...
Several biochemical tests were studied to determine their
correlation with coagulase activity. The lysozyme test was in perfect
agreement with the coagulase test for the strains studied.
Mannitol fermentation and egg yolk lipase tests were in agreement
with the coagulase test for 96% of the coagulase positive strains.
Sheep red...
Trypticase-Soy Broth (TSB) was inoculated with Streptococcus
diacetilactis, incubated at 21C for 24 hours and centrifuged to remove
the cells. When the spent TSB was inoculated with Staphylococcus
aureus, it allowed 99.8% less growth than fresh TSB. If the spent
TSB were supplemented with 0.08 μg/ml of niacinamide, this inhibition...
Previous studies have indicated that staphylococci have potential for use as indicators of water quality in swimming pool and other recreational waters. However, these organisms are not yet included in the official guidelines for recreational water quality promulgated by health authorities. The purpose of this study is to determine water...
The consumption of seafood in the United States has increased rapidly in recent years due to high quality protein and health benefits of seafood. Seafood can be a carrier for bacteria normally distributed in the marine environment and, in some cases, can be contaminated by human pathogens. Therefore, there is...
Mammalian skin is the largest organ of the body and serves as a barrier to protect the body against chemical, mechanical and pathogenic insults as well as water loss. The epidermis is formed from the primitive ectoderm into a multilayered stratified epithelium, consisting of basal, spinous, granular and outermost cornified...