The purpose of this study was to compare and test the efficiency of the leading kinds of media being used for the isolation and identification
of Clostridium botulinum type E strains, and to develop a
more reliable method and media for the differentiation of this organism
based on the "pearly...
During the development of the media for the identification and
differentiation of Clostridium perfringens and C. botulinum types A,
B and E, 16 different double layer media were prepared and tested
with known cultures of 28 C. botulinum strains, 20 C. perfringens
strains and 11 other clostridial species.
The semisolid...
In this study of toxigenic and non-toxigenic strains of Clostridium
botulinum type E, attempts were made to induce toxin production
in non-toxigenic cultures by exposure to mutagenic agents
and to differentiate the organisms by immunodiffusion. First, cultures
of non-toxic Cl. botulinum type E were exposed to various
mutagenic agents in...
There are six types of Cl. botulinum (Types A, B, C, D, E,
and F). The differentiation between these types is based upon the
antigenic structure of the toxin. Many strains of these various types
have been isolated. In addition, a number of nontoxigenic "type Elike"
strains have come to...
Symbiotic associations are established between non-leguminous
(actinorrhizal) nitrogen-fixing flowering plants and two categories
of microorganisms: mycorrhizal fungi and a filamentous actinomycete.
The actinomydete induces nodule formation and produces nitrogenase,
the enzyme responsible for the reduction of atmospheric nitrogen to
a form available to higher plants. The mycorrhizal fungus is found...