Strawberry is one of four crops included in the USDA-NIFA Specialty Crop Research Initiative-funded RosBREED project along with apple, peach, and cherry. Phenotyping strawberry for specific horticultural and commercial traits is important to identify associations with genotypic marker(s). This process is the first step in translating genomic knowledge into enhanced...
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2.2. Mean resistance scores anddisease response to Cdn-4 and Cdn-5 for
14 Fragaria
This dissertation describes the application and development of molecular tools that have great potential for use in studying variation in strawberry germplasm. The first study evaluated 91 microsatellite (simple sequence repeat, SSR) markers for transferability in 22 Fragaria species and their utility in fingerprinting octoploid strawberries. Out of the transferable...
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The level of polymorphisms of many biochemical and DNA markers are low in
cultivated sunflower (Helianthus annuus L.). The number of mapped public DNA
markers is limited. Molecular markers have not been developed for the most
important diseases of sunflower, such as downy mildew. The objectives of this
study were...
Molecular tools, coupled with unique germplasm stocks and rigorous phenotyping, are
useful for developing a better understanding of qualitative and quantitative disease resistance
genes in plants. The identification of molecular markers linked to all types of resistance
genes provides opportunities for implementing a range of resistance breeding strategies,
ranging from...
We tested a synthetic bacterio-opsin (bO) gene derived from Halobacterium halobium for its effect on disease resistance in transgenic poplar hybrids, and assessed a genetic system for controlling expression of this gene. In tobacco the bO gene caused necrotic lesions similar to an induced hypersensitive response (HR), increased levels of...
Genetic resistance is the most appropriate way to control diseases in crop plants. Resistance can be described as qualitative (involving a gene-for-gene system) or quantitative (with continuous distribution of disease levels). The relationships between different types of resistance remain obscure, as does the genetics of quantitative traits. The use of...
Quantitative resistance (QR) to disease is usually more durable than qualitative resistance, but its genetic basis is not well understood. We used the barley/barley stripe rust pathosystem as a model for the characterization of the QR phenotype and associated genomic regions. As an intermediate step in the preparation of near-isogenic...