Pseudomonas is a diverse genus of Gamma proteobacteria that are ubiquitous in the natural environment, including soil, water, plant surfaces, and animals. The Pseudomonas fluorescens group is a diverse collection of seven subgroups and more than 50 named species. This group is known for their production of a variety of...
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that is a member of the basic helix-loop-helix PER-ARNT-SIM (bHLH/PAS) protein family of cellular sensors. The AHR is conserved across multiple animal phyla and is required for proper vertebrate development. A structurally diverse group of chemicals, including polycyclic aromatic...
The focus of this dissertation was to understand protein function and structure ona molecular level. To do this successfully, a variety of biochemical and biophysicaltechniques were employed. A wide variety of techniques were applied to study theproteins of interest in this dissertation to probe protein-protein interactions, proteinfunction, protein structure, and...
Intrinsically disordered proteins (IDP) are a class of proteins that lack a three-dimensional structure and their prevalence and diverse functions in the cell have only been discovered relatively recently. The intermediate chain (IC) subunit of the microtubule motor protein complex dynein contains an N-terminal disordered region, N-IC, which is central...
Day 12-14 bovine embryos produce urokinase-type plasminogen activator (uPA) and
an uPA-plasminogen activator inhibitor (PAI) complex. The type of PAI, -1 or -2, in
the complex has not been determined therefore the objective of this research was to
identify the PAI produced by the Day 14 bovine embryo. In experiment...
The Hippo signaling pathway is an evolutionarily conserved regulator of cell growth, proliferation, and apoptosis. A key function of the pathway is to regulate the subcellular distribution and activity of Yorkie in Drosophila or Yes-associated protein (YAP) and transcription co-activator with PDZ-binding motif (TAZ) in mammals. The dysregulation of the...
The environmental health science community recognizes polycyclic aromatic hydrocarbons (PAHs) as a re-emerging class of environmental pollutants due to their persistence and prominence in mixtures of concern. Due to their widespread distribution in the environment, exposure to PAHs often occur as complex chemical mixtures. Exposures are linked to numerous adverse...
Circadian clocks coordinate molecular, cellular, physiological, and behavioral processes with the 24-hour solar day. While clock functions are well understood in young animals, it is not clear how aging or neurodegenerative disease affects the various levels of the circadian system. A common symptom of many neurodegenerative diseases including Alzheimer’s disease...
Skeletal muscle, the largest organ in the body in mass, is composed by 600 specialized muscles in humans with unique biochemical, physiological and metabolic identities. Skeletal muscles control body movement, locomotion, and nutrient balance. Muscle formation requires precisely orchestrated environmental signals and regulatory gene networks in time and space. Gene...
Cells represent microcosms of spatial and temporal structural organization, with the achievement of internal spatial organization relying upon a collection of macromolecular motor complexes to transport and localize components throughout the cell. Cytoplasmic dynein is one such motor complex, and is the principal ATP-dependent motor for retrograde transport along microtubules...